@article{oai:repo.qst.go.jp:00048165,
 author = {Isozaki, Tetsuro and Fujita, Mayumi and Yamada, Shigeru and Imadome, Kaori and Shoji, Yoshimi and Yasuda, Takeshi and Nakayama, Fumiaki and Imai, Takashi and Matsubara, Hisahiro and 磯崎 哲朗 and 藤田 真由美 and 山田 滋 and 今留 香織 and 荘司 好美 and 安田 武嗣 and 中山 文明 and 今井 高志 and 松原 久裕},
 issue = {1},
 journal = {International journal of oncology},
 month = {Jul},
 note = {C-ion radiotherapy is associated with improved local control and survival in several types of tumors. Although C-ion irradiation is widely reported to effectively induce DNA damage in tumor cells, the effects of irradiation on proteins, such as protein stability or degradation in response to radiation stress, remain unknown. We aimed to compare the effects of C-ion and X-ray irradiation focusing on the cellular accumulation of ubiquitylated proteins. Cells from two human colorectal cancer cell lines, SW620 and SW480, were subjected to C-ion or X-ray irradiation and determination of ubiquitylated protein levels. High levels of ubiquitylated protein accumulation were observed in the C-ion-irradiated SW620 with a peak at 3 Gy; the accumulation was significantly lower in the X-ray-irradiated SW620 at all doses. Enhanced levels of ubiquitylated proteins were also detected in C-ion or X-ray-irradiated SW480, however, those levels were significantly lower than the peak detected in the C-ion-irradiated SW620. The levels of irradiation-induced ubiquitylated proteins decreased in a time-dependent manner, suggesting that the proteins were eliminated after irradiation. The treatment of C-ion-irradiated SW620 with a proteasome inhibitor (epoxomicin) enhanced the cell killing activity. The accumulated ubiquitylated proteins were co-localized with γ-H2AX, and with TP53BP1, in C-ion-irradiated SW620, indicating C-ion-induced ubiquitylated proteins may have some functions in the DNA repair system. Overall, we showed C-ion irradiation strongly induces the accumulation of ubiquitylated proteins in SW620. These characteristics may play a role in improving the therapeutic ratio of C-ion beams; blocking the clearance of ubiquitylated proteins may enhance sensitivity to C-ion radiation.},
 pages = {144--152},
 title = {Effects of carbon ion irradiation and X-ray irradiation on the ubiquitylated protein accumulation.},
 volume = {49},
 year = {2016}
}