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Establishment of a DEN-induced mouse model of esophageal squamous cell carcinoma metastasis
https://repo.qst.go.jp/records/48143
https://repo.qst.go.jp/records/4814354e613e7-9912-4732-9ded-350a46991f05
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-07-24 | |||||
| タイトル | ||||||
| タイトル | Establishment of a DEN-induced mouse model of esophageal squamous cell carcinoma metastasis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
成島, 一夫
× 成島, 一夫× 島田, 英昭× 松原, 久裕× 山田, 滋× 柿沼, 志津子× 森岡, 孝満× 島田, 義也× 成島 一夫× 島田 英昭× 松原 久裕× 山田 滋× 柿沼 志津子× 森岡 孝満× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Syngeneic transplantation mouse models have been used to evaluate the efficacy of immunotherapy in addition to radiotherapy and chemotherapy for treating cancer. However, the mouse models of esophageal squamous cell carcinoma have yet to be established. Therefore, we aimed to develop a mouse model of esophageal squamous cell carcinoma. Methods: Male and female Balb/c, C3H, and C57BL mice received diethyl nitrosamine continuously for 4 months. On completion of the 200-day treatment period, animals were killed, and esophageal, forestomach, lung, and liver samples were examined macroscopically and by the histopathological analysis. Induced tumors from C3H female mice were mechanically dissociated into small pieces and were mixed with the brain homogenates, and injected into interscapular region subcutaneously into syngeneic C3H female mice to evaluate tumor growth and/or metastatic potential. Results: The incidence of esophageal/forestomach squamous cell carcinoma varied according to mouse strain and gender, and the C3H mouse was found to be most susceptible. Pathologically, tumors were predominantly well-differentiated squamous cell carcinoma, with a proportion of tumors developing distant metastases. Transplanted esophageal squamous cell carcinoma cells developed subcutaneous tumors in syngeneic mice, with distant metastases into the lung. Metastatic tumors had poorly differentiated components histologically with Ki-67 and p53 expression. Metastatic tumors had lower p21 expression than transplanted tumors. Conclusion: In the present study, we demonstrate the establishment of a mouse model esophageal squamous cell carcinoma allowing transplantation into syngeneic mice with distant metastatic potential. We believe that the present syngeneic mouse model will have utility in various preclinical research fields, including cancer immunotherapy. |
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| 書誌情報 |
Esophagus 巻 14, 号 2, p. 131-137, 発行日 2016-09 |
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| 出版者 | ||||||
| 出版者 | Springer Japan | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1612-9059 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s10388-016-0555-3 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://link.springer.com/article/10.1007/s10388-016-0555-3 | |||||
| 関連名称 | http://link.springer.com/article/10.1007/s10388-016-0555-3 | |||||
