@article{oai:repo.qst.go.jp:00048143,
 author = {成島, 一夫 and 島田, 英昭 and 松原, 久裕 and 山田, 滋 and 柿沼, 志津子 and 森岡, 孝満 and 島田, 義也 and 成島 一夫 and 島田 英昭 and 松原 久裕 and 山田 滋 and 柿沼 志津子 and 森岡 孝満 and 島田 義也},
 issue = {2},
 journal = {Esophagus},
 month = {Sep},
 note = {Background: Syngeneic transplantation mouse models have been used to evaluate the efficacy of immunotherapy in addition to radiotherapy and chemotherapy for treating cancer. However, the mouse models of esophageal squamous cell carcinoma have yet to be established. Therefore, we aimed to develop a mouse model of esophageal squamous cell carcinoma.
Methods: Male and female Balb/c, C3H, and C57BL mice received diethyl nitrosamine continuously for 4 months. On completion of the 200-day treatment period, animals were killed, and esophageal, forestomach, lung, and liver samples were examined macroscopically and by the histopathological analysis. Induced tumors from C3H female mice were mechanically dissociated into small pieces and were mixed with the brain homogenates, and injected into interscapular region subcutaneously into syngeneic C3H female mice to evaluate tumor growth and/or metastatic potential.
Results: The incidence of esophageal/forestomach squamous cell carcinoma varied according to mouse strain and gender, and the C3H mouse was found to be most susceptible. Pathologically, tumors were predominantly well-differentiated squamous cell carcinoma, with a proportion of tumors developing distant metastases. Transplanted esophageal squamous cell carcinoma cells developed subcutaneous tumors in syngeneic mice, with distant metastases into the lung. Metastatic tumors had poorly differentiated components histologically with Ki-67 and p53 expression. Metastatic tumors had lower p21 expression than transplanted tumors.
Conclusion: In the present study, we demonstrate the establishment of a mouse model esophageal squamous cell carcinoma allowing transplantation into syngeneic mice with distant metastatic potential. We believe that the present syngeneic mouse model will have utility in various preclinical research fields, including cancer immunotherapy.},
 pages = {131--137},
 title = {Establishment of a DEN-induced mouse model of esophageal squamous cell carcinoma metastasis},
 volume = {14},
 year = {2016}
}