WEKO3
アイテム
Elemental and immunohistochemical analysis of the lungs and hilar lymph node in a patient with asbestos exposure, a pilot study
https://repo.qst.go.jp/records/47913
https://repo.qst.go.jp/records/47913f72f35d6-6095-4f9d-930c-e4a968e0a6e4
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-05-01 | |||||
| タイトル | ||||||
| タイトル | Elemental and immunohistochemical analysis of the lungs and hilar lymph node in a patient with asbestos exposure, a pilot study | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Koga, Yasuhiko
× Koga, Yasuhiko× Satoh, Takahiro× Kaira, Kyoichi× Koka, Masashi× Hisada, Takeshi× Hirato, Junko× Altan, Bolag× Yatomi, Masakiyo× Ono, Akihiro× Kamide, Yosuke× Shimizu, Yasuo× Aoki-Saito, Haruka× Tsurumaki, Hiroaki× Shimizu, Kimihiro× Mogi, Akira× Ishizuka, Tamotsu× Yamada, Masanobu× Dobashi, Kunio× 佐藤 隆博 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Studies have shown that inhaled mine dust, such as asbestos, can be translocated to various organs including the lymph nodes. Recently, we have established a protocol that enables us to identify inhaled elements using paraffin embedded lung specimens by in-air microparticle-induced X-ray emission (micro-PIXE). However, little research has examined the concentration of these inhaled fibers in various organs or the mechanisms of their translocation. In this study, we compared the concentration of inhaled fibers in the lung parenchyma to the concentration in the hilar lymph node as well as to determine the elemental spatial distribution of the inhaled fibers in a patient with occupational asbestos exposure. Lung tissues and hilar lymph node in a patient with asbestos exposure were used in this study. Elemental analysis was performed by in-air micro-PIXE. Immunohistochemical analysis was performed using anti CD163, smooth muscle actin, vimentin and β-catenin antibody. The analysis revealed that the amount of inhaled silicon was approximately 6 times higher in the lymph node than in the lungs. The spatial analysis showed that silicon, iron and aluminium were co-localized in the hilar lymph node. The immunohistochemical analysis showed localized agreement of the inhaled fibers with macrophages, smooth muscle actin, and vimentin in the hilar lymph node. This study showed that in-air micro-PIXE could be useful for analyzing the elemental distribution and quantification of inhaled fibers in the human body. Furthermore, immunohistochemistry in combination with in-air micro-PIXE analyses may help to determine the mechanism of mine dust distribution in vivo. |
|||||
| 書誌情報 |
Environmental Health and Preventive Medicine 巻 21, 号 6, p. 492-500, 発行日 2016-11 |
|||||
| 出版者 | ||||||
| 出版者 | Springer International Publishing AG. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1342-078X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1007/s12199-016-0576-5 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s12199-016-0576-5 | |||||
| 関連名称 | https://doi.org/10.1007/s12199-016-0576-5 | |||||
