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Multimodal Imaging for DREADD-expressing Neurons in Living Brain and Their Application to Implantation of iPSC-derived Neural Progenitors
https://repo.qst.go.jp/records/47777
https://repo.qst.go.jp/records/4777736fd58f4-b118-4e3e-85cd-612450975b5a
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-04-25 | |||||
| タイトル | ||||||
| タイトル | Multimodal Imaging for DREADD-expressing Neurons in Living Brain and Their Application to Implantation of iPSC-derived Neural Progenitors | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
JI, BIN
× JI, BIN× Kaneko, Hiroyuki× Minamimoto, Takafumi× Inoue, Haruhisa× Takeuchi, Hiroki× Kumata, Katsushi× Zhang, Ming-Rong× Aoki, Ichio× Seki, Chie× Ono, Maiko× Tokunaga, Masaki× Tsukamoto, Satoshi× Tanabe, Koji× Shin, Ryong-Moon× Minamihisamatsu, Takeharu× Kito, Seiji× J., Richmond Barry× Suhara, Tetsuya× Higuchi, Makoto× 季 斌× 南本 敬史× 熊田 勝志× 張 明栄× 青木 伊知男× 関 千江× 小野 麻衣子× 徳永 正希× 塚本 智史× 南久松 丈晴× 鬼頭 靖司× 須原 哲也× 樋口 真人 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Chemogenetic manipulation of neuronal activities has been enabled by a designer receptor (DREADD) exclusively activated by clozapine-N-oxide (CNO). Here, we applied CNO as a functional reporter probe to positron emission tomography (PET) of DREADD in living brains. Mutant human M4 DREADD (hM4Di) expressed in transgenic mouse neurons was visualized by PET with microdose [11C]CNO. Deactivation of DREADD-expressing neurons in these mice by nonradioactive CNO at a pharmacological dose could also be captured by arterial spin labeling MRI (ASL-MRI). Neuroprogenitors derived from hM4Di transgenic-induced pluripotent stem cells (iPSCs) were then implanted into wild-type mouse brains, and neuronal differentiation of the grafts could be imaged by [11C]CNO-PET. Finally, ASL-MRI captured chemogenetic functional manipulation of the graft neurons. Our data provide the first demonstration of multimodal molecular/functional imaging of cells expressing a functional gene reporter in the brain, which would be translatable to humans for therapeutic gene transfers and cell replacements. | |||||
| 書誌情報 |
The Journal of neuroscience : the official journal of the Society for Neuroscience 巻 36, 号 45, p. 11544-11558, 発行日 2016-11 |
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| 出版者 | ||||||
| 出版者 | Society for Neuroscience | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 27911758 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1523/JNEUROSCI.1279-16.2016 | |||||
