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Metformin enhances the radiosensitivity of human liver cancer cells to γ–rays and carbon ion beams
https://repo.qst.go.jp/records/47641
https://repo.qst.go.jp/records/4764119482136-c61d-46b2-9eb7-8fd0f6ba6abd
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-02-15 | |||||
| タイトル | ||||||
| タイトル | Metformin enhances the radiosensitivity of human liver cancer cells to γ–rays and carbon ion beams | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
EunHo, Kim
× EunHo, Kim× Mi-Sook, Kim× 古澤, 佳也× 鵜澤, 玲子× Soorim, Han× Won-Gyun, Jung× 崔, 星× Ｋｉｍ Ｅｕｎ Ｈｏ× 古澤 佳也× 鵜澤 玲子× 崔 星 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | ABSTRACT Purpose: To investigate the effect of metformin on the responses of hepatocellular carcinoma (HCC) cells to γ–rays (low-linear energy transfer (LET) radiation) and carbon-ion beams (high-LET radiation). Methods: Huh-7 and HepG2 cells were pretreated with metformin and exposed to a single dose of fraction γ–rays or carbon ion beams (13 and 70keV/μm). Cell survival, DNA repair, apoptosis, cell cycle progression, migration, invasion and the AMPK/mTOR/Akt signaling pathway were assessed. Results: Irradiation with γ-rays and carbon ion beams decreased the clonogenic survival of HCC cells in a dose-dependent manner, and metformin significantly increased radiation-induced clonogenic cell death. The relative biological effectiveness (RBE) of Huh7 (HepG2) cells at 13 and 70keV/μm carbon ion beams relative to γ-rays at the D10 levels was approximately 1.68 (1.11) and 2.37 (2.00), respectively. The γH2AX and comet assays showed that 70keV/μm carbon ion beams induced DNA damage more effectively than 13keV/μm carbon ion beams. Metformin significantly enhanced the DNA damage caused by irradiation, particularly that of 70keV/μm carbon ion beams. Higher levels of apoptosis were induced by 70keV/μm carbon ion beams than by 13keV/μm carbon ion beams and γ-rays, and metformin increased radiation-induced apoptosis. Carbon ion beams combined with metformin more effectively induced subG1 and G2/M arrest, reduced the expression of N-cadherin and vimentin, enhanced phospho-AMPK expression and downregulated phospho-mTOR and phospho-Akt than carbon ion beams or γ-rays alone. |
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| 書誌情報 |
Oncotarget 巻 49, 号 7, p. 80568-80578, 発行日 2016-10 |
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| 出版者 | ||||||
| 出版者 | Impact Journals, LLC | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1949-2553 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 27802188 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.18632/oncotarget.12966 | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=12966&pubmed-linkout=1 | |||||
| 関連名称 | http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=12966&pubmed-linkout=1 | |||||
