{"created":"2023-05-15T14:36:58.175800+00:00","id":47641,"links":{},"metadata":{"_buckets":{"deposit":"959a7235-f761-443a-8dab-5c35651b3cda"},"_deposit":{"created_by":1,"id":"47641","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47641"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047641","sets":["1"]},"author_link":["477773","477782","477775","477780","477783","477779","477776","477777","477778","477774","477781"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"80578","bibliographicPageStart":"80568","bibliographicVolumeNumber":"49","bibliographic_titles":[{"bibliographic_title":"Oncotarget"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ABSTRACT\nPurpose: To investigate the effect of metformin on the responses of hepatocellular carcinoma (HCC) cells to γ–rays (low-linear energy transfer (LET) radiation) and carbon-ion beams (high-LET radiation).\nMethods: Huh-7 and HepG2 cells were pretreated with metformin and exposed to a single dose of fraction γ–rays or carbon ion beams (13 and 70keV/μm). Cell survival, DNA repair, apoptosis, cell cycle progression, migration, invasion and the AMPK/mTOR/Akt signaling pathway were assessed.\nResults: Irradiation with γ-rays and carbon ion beams decreased the clonogenic survival of HCC cells in a dose-dependent manner, and metformin significantly increased radiation-induced clonogenic cell death. The relative biological effectiveness (RBE) of Huh7 (HepG2) cells at 13 and 70keV/μm carbon ion beams relative to γ-rays at the D10 levels was approximately 1.68 (1.11) and 2.37 (2.00), respectively.\nThe γH2AX and comet assays showed that 70keV/μm carbon ion beams induced DNA damage more effectively than 13keV/μm carbon ion beams. Metformin significantly enhanced the DNA damage caused by irradiation, particularly that of 70keV/μm carbon ion beams. Higher levels of apoptosis were induced by 70keV/μm carbon ion beams than by 13keV/μm carbon ion beams and γ-rays, and metformin increased radiation-induced apoptosis. Carbon ion beams combined with metformin more effectively induced subG1 and G2/M arrest, reduced the expression of N-cadherin and vimentin, enhanced phospho-AMPK expression and downregulated phospho-mTOR and phospho-Akt than carbon ion beams or γ-rays alone.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Impact Journals, LLC"}]},"item_8_relation_13":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"27802188","subitem_relation_type_select":"PMID"}}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.18632/oncotarget.12966","subitem_relation_type_select":"DOI"}}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=12966&pubmed-linkout=1"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path[]=12966&pubmed-linkout=1","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1949-2553","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"EunHo, Kim"}],"nameIdentifiers":[{"nameIdentifier":"477773","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mi-Sook, Kim"}],"nameIdentifiers":[{"nameIdentifier":"477774","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤, 佳也"}],"nameIdentifiers":[{"nameIdentifier":"477775","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鵜澤, 玲子"}],"nameIdentifiers":[{"nameIdentifier":"477776","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Soorim, Han"}],"nameIdentifiers":[{"nameIdentifier":"477777","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Won-Gyun, Jung"}],"nameIdentifiers":[{"nameIdentifier":"477778","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔, 星"}],"nameIdentifiers":[{"nameIdentifier":"477779","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ｋｉｍ Ｅｕｎ Ｈｏ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"477780","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古澤 佳也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"477781","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鵜澤 玲子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"477782","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"崔 星","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"477783","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Metformin enhances the radiosensitivity of human liver cancer cells to γ–rays and carbon ion beams","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Metformin enhances the radiosensitivity of human liver cancer cells to γ–rays and carbon ion beams"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-02-15"},"publish_date":"2017-02-15","publish_status":"0","recid":"47641","relation_version_is_last":true,"title":["Metformin enhances the radiosensitivity of human liver cancer cells to γ–rays and carbon ion beams"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:37:16.042125+00:00"}