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Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells.
https://repo.qst.go.jp/records/47498
https://repo.qst.go.jp/records/4749801376743-8edf-497d-b431-547c15b3fa25
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-08-24 | |||||
| タイトル | ||||||
| タイトル | Sendai virus-mediated expression of reprogramming factors promotes plasticity of human neuroblastoma cells. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Islam, Rafiqul
× Islam, Rafiqul× Suenaga, Y× Takatori, A× al., et× イスラム ラフィクル |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Neuroblastoma (NB) is the most common extracranial solid tumor that originates from multipotent neural crest cells. NB cell populations which express embryonic stem cell-associated genes have been identified and shown to retain a multipotent phenotype. However, whether somatic reprogramming of NB cells can produce similar stem-cell like populations is unknown. Here, we sought to reprogram NB cell lines using an integration-free Sendai virus vector system. Of four NB cell lines examined, only SH-IN cells formed induced pluripotent stem cell-like colonies (SH-IN 4F colonies) at approximately 6 weeks following transduction. These SH-IN 4F colonies were alkaline phosphatase-positive. Array comparative genomic hybridization analysis indicated identical genomic aberrations in the SH-IN 4F cells as in the parental cells. SH-IN 4F cells had the ability to differentiate into the three embryonic germ layers in vitro, but rather formed NBs in vivo. Furthermore, SH-IN 4F cells exhibited resistance to cisplatin treatment and differentiated into endothelial-like cells expressing CD31 in the presence of vascular endothelial growth factor. These results suggest that SH-IN 4F cells are partially reprogrammed NB cells, and could be a suitable model for investigating the plasticity of aggressive tumors. This article is protected by copyright. All rights reserved. | |||||
| 書誌情報 |
Cancer Science 巻 106, 号 10, p. 1351-1361, 発行日 2015-08 |
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| 出版者 | ||||||
| 出版者 | WILEY | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1111/cas.12746 | |||||
