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Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation
https://repo.qst.go.jp/records/47402
https://repo.qst.go.jp/records/47402637cfc3c-201b-4ad4-8d43-47db2f97f79f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-05-18 | |||||
| タイトル | ||||||
| タイトル | Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Fujita, Mayumi
× Fujita, Mayumi× Imadome, Kaori× Shoji, Yoshimi× Isozaki, Teturou× Endo, Satoshi× Yamada, Shigeru× Imai, Takashi× 藤田 真由美× 今留 香織× 荘司 好美× 磯崎 哲朗× 遠藤 悟史× 山田 滋× 今井 高志 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Purpose This study investigated the mechanisms underlying the inhibition of cancer cell migration and invasion by carbon (C)-ion irradiation. \nMethods and Materials Human pancreatic cancer cells MIAPaCa-2, AsPC-1, and BxPC-3 were treated by X-ray (4 Gy) or C-ion (0.5, 1, 2, or 4 Gy) irradiation, and their migration and invasion were assessed 2 days later. The levels of GTP-bound Rac1 and RhoA were determined by the active GTPase pull-down assay with or without a proteasome inhibitor, and the binding of E3 ubiquitin ligase to GTP-bound Rac1 was examined by immunoprecipitation. \nResults C-ion irradiation reduced the levels of GTP-bound Rac1 and RhoA, two major regulators of cell motility, in MIAPaCa-2 cells and GTP-bound Rac1 in AsPC-1 and BxPC-3 cells. Proteasome inhibition reversed the effect, indicating that C-ion irradiation induced Rac1 and RhoA degradation via the ubiquitin (Ub)-proteasome pathway. E3 Ub ligase X-linked inhibitor of apoptosis protein (XIAP) which directly targets Rac1, was selectively induced in C-ion irradiated MIAPaCa-2 cells and co-precipitated with GTP-bound Rac1 in C-ion-irradiated cells, which was associated with Rac1 ubiquitination. Cell migration and invasion reduced by C-ion radiation were restored by siRNA-mediated XIAP knockdown, indicating that XIAP is involved in C-ion-induced inhibition of cell motility. \nConclusion In contrast to X-ray irradiation, C-ion treatment inhibited the activity of Rac1 and RhoA in MIAPaCa-2 cells and Rac1 in AsPC-1 and BxPC-3 cells via Ub-mediated proteasomal degradation, thereby blocking the motility of these pancreatic cancer cells. |
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| 書誌情報 |
International Journal of Radiation Oncology Biology Physics 巻 93, 号 1, p. 173-180, 発行日 2015-05 |
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| 出版者 | ||||||
| 出版者 | Tarrytown Ny : Elsevier Science Inc | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0360-3016 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | doi:10.1016/j.ijrobp.2015.05.009 | |||||
