{"created":"2023-05-15T14:36:47.501705+00:00","id":47402,"links":{},"metadata":{"_buckets":{"deposit":"ef051943-fdfb-496f-9ffe-2a6eb63518b0"},"_deposit":{"created_by":1,"id":"47402","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47402"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047402","sets":["1"]},"author_link":["474627","474625","474616","474621","474618","474622","474617","474614","474623","474620","474615","474626","474624","474619"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"180","bibliographicPageStart":"173","bibliographicVolumeNumber":"93","bibliographic_titles":[{"bibliographic_title":"International Journal of Radiation Oncology Biology Physics"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose\nThis study investigated the mechanisms underlying the inhibition of cancer cell migration and invasion by carbon (C)-ion irradiation.\n\\nMethods and Materials\nHuman pancreatic cancer cells MIAPaCa-2, AsPC-1, and BxPC-3 were treated by X-ray (4 Gy) or C-ion (0.5, 1, 2, or 4 Gy) irradiation, and their migration and invasion were assessed 2 days later. The levels of GTP-bound Rac1 and RhoA were determined by the active GTPase pull-down assay with or without a proteasome inhibitor, and the binding of E3 ubiquitin ligase to GTP-bound Rac1 was examined by immunoprecipitation.\n\\nResults\nC-ion irradiation reduced the levels of GTP-bound Rac1 and RhoA, two major regulators of cell motility, in MIAPaCa-2 cells and GTP-bound Rac1 in AsPC-1 and BxPC-3 cells. Proteasome inhibition reversed the effect, indicating that C-ion irradiation induced Rac1 and RhoA degradation via the ubiquitin (Ub)-proteasome pathway. E3 Ub ligase X-linked inhibitor of apoptosis protein (XIAP) which directly targets Rac1, was selectively induced in C-ion irradiated MIAPaCa-2 cells and co-precipitated with GTP-bound Rac1 in C-ion-irradiated cells, which was associated with Rac1 ubiquitination. Cell migration and invasion reduced by C-ion radiation were restored by siRNA-mediated XIAP knockdown, indicating that XIAP is involved in C-ion-induced inhibition of cell motility.\n\\nConclusion\nIn contrast to X-ray irradiation, C-ion treatment inhibited the activity of Rac1 and RhoA in MIAPaCa-2 cells and Rac1 in AsPC-1 and BxPC-3 cells via Ub-mediated proteasomal degradation, thereby blocking the motility of these pancreatic cancer cells.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Tarrytown Ny : Elsevier Science Inc"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"doi:10.1016/j.ijrobp.2015.05.009","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0360-3016","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujita, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"474614","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imadome, Kaori"}],"nameIdentifiers":[{"nameIdentifier":"474615","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shoji, Yoshimi"}],"nameIdentifiers":[{"nameIdentifier":"474616","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Isozaki, Teturou"}],"nameIdentifiers":[{"nameIdentifier":"474617","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Endo, Satoshi"}],"nameIdentifiers":[{"nameIdentifier":"474618","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamada, Shigeru"}],"nameIdentifiers":[{"nameIdentifier":"474619","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Imai, Takashi"}],"nameIdentifiers":[{"nameIdentifier":"474620","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤田 真由美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474621","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今留 香織","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474622","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荘司 好美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474623","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"磯崎 哲朗","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474624","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"遠藤 悟史","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474625","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山田 滋","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474626","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今井 高志","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"474627","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation ","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation "}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-18"},"publish_date":"2016-05-18","publish_status":"0","recid":"47402","relation_version_is_last":true,"title":["Carbon-ion irradiation suppresses migration and invasiveness of human pancreatic carcinoma cells MIAPaCa-2 via Rac1 and RhoA degradation "],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:40:17.163881+00:00"}