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Radiation increases the cellular uptake of exosomes through CD29/CD81 complex formation.
https://repo.qst.go.jp/records/47177
https://repo.qst.go.jp/records/4717734f3bbe0-c51f-4d59-84db-2abfe50abcd6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-07-23 | |||||
| タイトル | ||||||
| タイトル | Radiation increases the cellular uptake of exosomes through CD29/CD81 complex formation. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hazawa, Masaharu
× Hazawa, Masaharu× Tomiyama, Kenichi× Saotome-Nakamura, Ai× Obara, Chizuka× Yasuda, Takeshi× Gotoh, Takaya× Tanaka, Izumi× Yakumaru, Haruko× Ishihara, Hiroshi× Tajima, Katsushi× 羽澤 勝治× 富山 健一× 早乙女 愛× 小原 千寿香× 安田 武嗣× 後藤 孝也× 田中 泉× 薬丸 晴子× 石原 弘× 田嶋 克史 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Exosomes mediate intercellular communication, and mesenchymal stem cells (MSC) or their secreted exosomes affect a number of pathophysiologic states. Clinical applications of MSC and exosomes are increasingly anticipated. Radiation therapy is the main therapeutic tool for a number of various conditions. The cellular uptake mechanisms of exosomes and the effects of radiation on exosome-cell interactions are crucial, but they are not well understood. Here we examined the basic mechanisms and effects of radiation on exosome uptake processes in MSC. Radiation increased the cellular uptake of exosomes. Radiation markedly enhanced the initial cellular attachment to exosomes and induced the colocalization of integrin CD29 and tetraspanin CD81 on the cell surface without affecting their expression levels. Exosomes dominantly bound to the CD29/CD81 complex. Knockdown of CD29 completely inhibited the radiation-induced uptake, and additional or single knockdown of CD81 inhibited basal uptake as well as the increase in radiation-induced uptake. We also examined possible exosome uptake processes affected by radiation. Radiation-induced changes did not involve dynamin2, reactive oxygen species, or their evoked p38 mitogen-activated protein kinase-dependent endocytic or pinocytic pathways. Radiation increased the cellular uptake of exosomes through CD29/CD81 complex formation. These findings provide essential basic insights for potential therapeutic applications of exosomes or MSC in combination with radiation. | |||||
| 書誌情報 |
Biochemical and biophysical research communications 巻 446, 号 4, p. 1165-1171, 発行日 2014-01 |
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| 出版者 | ||||||
| 出版者 | Academic Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006-291X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 24667602 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.bbrc.2014.03.067 | |||||
