@article{oai:repo.qst.go.jp:00047177,
 author = {Hazawa, Masaharu and Tomiyama, Kenichi and Saotome-Nakamura, Ai and Obara, Chizuka and Yasuda, Takeshi and Gotoh, Takaya and Tanaka, Izumi and Yakumaru, Haruko and Ishihara, Hiroshi and Tajima, Katsushi and 羽澤 勝治 and 富山 健一 and 早乙女 愛 and 小原 千寿香 and 安田 武嗣 and 後藤 孝也 and 田中 泉 and 薬丸 晴子 and 石原 弘 and 田嶋 克史},
 issue = {4},
 journal = {Biochemical and biophysical research communications},
 month = {Jan},
 note = {Exosomes mediate intercellular communication, and mesenchymal stem cells (MSC) or their secreted exosomes affect a number of pathophysiologic states. Clinical applications of MSC and exosomes are increasingly anticipated. Radiation therapy is the main therapeutic tool for a number of various conditions. The cellular uptake mechanisms of exosomes and the effects of radiation on exosome-cell interactions are crucial, but they are not well understood. Here we examined the basic mechanisms and effects of radiation on exosome uptake processes in MSC. Radiation increased the cellular uptake of exosomes. Radiation markedly enhanced the initial cellular attachment to exosomes and induced the colocalization of integrin CD29 and tetraspanin CD81 on the cell surface without affecting their expression levels. Exosomes dominantly bound to the CD29/CD81 complex. Knockdown of CD29 completely inhibited the radiation-induced uptake, and additional or single knockdown of CD81 inhibited basal uptake as well as the increase in radiation-induced uptake. We also examined possible exosome uptake processes affected by radiation. Radiation-induced changes did not involve dynamin2, reactive oxygen species, or their evoked p38 mitogen-activated protein kinase-dependent endocytic or pinocytic pathways. Radiation increased the cellular uptake of exosomes through CD29/CD81 complex formation. These findings provide essential basic insights for potential therapeutic applications of exosomes or MSC in combination with radiation.},
 pages = {1165--1171},
 title = {Radiation increases the cellular uptake of exosomes through CD29/CD81 complex formation.},
 volume = {446},
 year = {2014}
}