WEKO3
アイテム
Gene regulation by intracellular delivery and photodegradation of nanoparticles containing small interfering RNA.
https://repo.qst.go.jp/records/47109
https://repo.qst.go.jp/records/47109b391c3e9-79fb-4886-a589-91198cbc9955
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-04-27 | |||||
| タイトル | ||||||
| タイトル | Gene regulation by intracellular delivery and photodegradation of nanoparticles containing small interfering RNA. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Murayama, Shuhei
× Murayama, Shuhei× Kos, Petra× Miyata, Kanjiro× Kataoka, Kazunori× Wagner, Ernst× Kato, Masaru× 村山 周平× 加藤 大 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Although the use of small interfering RNA (siRNA) is a promising technique for gene regulation, spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, a method for spatiotemporal regulation of genes with nanoparticles containing siRNA is reported. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3-Luc cells, where the siRNA is released from the nanoparticles by UV irradiation for 30 s. The encapsulated siRNA only shows no gene-silencing effects, but release of the siRNA upon UV radiation leads to sequence-specific silencing of the luciferase gene in the cells. These results indicate that photodegradable siRNA-containing nanoparticles can be useful for time- and space-dependent regulation of gene expression in cells. | |||||
| 書誌情報 |
Macromolecular bioscience 巻 14, 号 5, p. 626-631, 発行日 2014-05 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1616-5187 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 24510781 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/mabi.201300393 | |||||
