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PCR primers for assessing community structure of aquatic fungi including Chytridiomycota and Cryptomycota
https://repo.qst.go.jp/records/47051
https://repo.qst.go.jp/records/470517c80cb5d-e83c-4d78-8e66-5a40a54de7e5
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-03-25 | |||||
| タイトル | ||||||
| タイトル | PCR primers for assessing community structure of aquatic fungi including Chytridiomycota and Cryptomycota | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
石井, 伸昌
× 石井, 伸昌× 石田, 聖二× 鏡味, 麻衣子× 石井 伸昌 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Fungi are diverse and have the potential for material cycling in freshwater ecosystems. Structural dynamics of aquatic fungi are often key to their role in the functioning of the ecosystems. However, community structure of aquatic fungi, especially for Chytridiomycota (chytrid) and Cryptomycota, is still limited because few primer sets are available to examine species composition. In this study, we validated six primer sets for the detection of aquatic fungi by denaturing gradient gel electrophoresis (DGGE) analysis and found that FF390W/EF3r showed the highest specificity among the primer sets tested. We detected both chytrids and Cryptomycota from Lake Inba by DGGE analysis using the FF390W/EF3r-GC primer set. Further study with our established analysis revealed community structures of aquatic fungi and their seasonal succession patterns in the lake. Results of our study are useful for selection of the primer set for community structures of aquatic fungi and their seasonal succession by DGGE analysis. | |||||
| 書誌情報 |
Fungal Ecology 巻 13, p. 33-43, 発行日 2015-02 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1754-5048 | |||||
