{"created":"2023-05-15T14:36:31.964033+00:00","id":47051,"links":{},"metadata":{"_buckets":{"deposit":"0c2517ae-300a-4f4e-b431-ab3df0934cea"},"_deposit":{"created_by":1,"id":"47051","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"47051"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00047051","sets":["1"]},"author_link":["469826","469827","469825","469824"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-02","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"43","bibliographicPageStart":"33","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"Fungal Ecology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Fungi are diverse and have the potential for material cycling in freshwater ecosystems.  Structural dynamics of aquatic fungi are often key to their role in the functioning of the ecosystems. However, community structure of aquatic fungi, especially for Chytridiomycota (chytrid) and Cryptomycota, is still limited because few primer sets are available to examine species composition.  In this study, we validated six primer sets for the detection of aquatic fungi by denaturing gradient gel electrophoresis (DGGE) analysis and found that FF390W/EF3r showed the highest specificity among the primer sets tested.  We detected both chytrids and Cryptomycota from Lake Inba by DGGE analysis using the FF390W/EF3r-GC primer set.  Further study with our established analysis revealed community structures of aquatic fungi and their seasonal succession patterns in the lake.  Results of our study are useful for selection of the primer set for community structures of aquatic fungi and their seasonal succession by DGGE analysis.","subitem_description_type":"Abstract"}]},"item_8_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1754-5048","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石井, 伸昌"}],"nameIdentifiers":[{"nameIdentifier":"469824","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石田, 聖二"}],"nameIdentifiers":[{"nameIdentifier":"469825","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鏡味, 麻衣子"}],"nameIdentifiers":[{"nameIdentifier":"469826","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"石井 伸昌","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"469827","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"PCR primers for assessing community structure of aquatic fungi including Chytridiomycota and Cryptomycota","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"PCR primers for assessing community structure of aquatic fungi including Chytridiomycota and Cryptomycota"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-03-25"},"publish_date":"2015-03-25","publish_status":"0","recid":"47051","relation_version_is_last":true,"title":["PCR primers for assessing community structure of aquatic fungi including Chytridiomycota and Cryptomycota"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:44:29.535591+00:00"}