WEKO3
アイテム
Visualisation of gH2AX Foci Caused by Heavy Ion Particle Traversal; Distinction between Core Track versus Non- Track Damage
https://repo.qst.go.jp/records/46955
https://repo.qst.go.jp/records/4695572e8310c-a045-428f-aba9-08c601aa70df
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-19 | |||||
| タイトル | ||||||
| タイトル | Visualisation of gH2AX Foci Caused by Heavy Ion Particle Traversal; Distinction between Core Track versus Non- Track Damage | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nakajima, Nakako
× Nakajima, Nakako× Holly, Brunton× Watanabe, Ritsuko× Hirayama, Ryoichi× Matsufuji, Naruhiro× Fujimori, Akira× Murakami, Takeshi× Okayasu, Ryuichi× Jeggo, Penny× Shibata, Atsushi× et.al× 中島 菜花子× Ｈｏｌｌｙ Ｂｒｕｎｔｏｎ× 平山 亮一× 松藤 成弘× 藤森 亮× 村上 健× 岡安 隆一× 柴田 淳史 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Heavy particle irradiation produces complex DNA double strand breaks (DSBs) which can arise from primary ionisation events within the particle trajectory. Additionally, secondary electrons, termed delta-electrons, which have a range of distributions can create low linear energy transfer (LET) damage within but also distant from the track. DNA damage by delta-electrons distant from the track has not previously been carefully characterised. Using imaging with deconvolution, we show that at 8 hours after exposure to Fe (~200 keV/um) ions, gH2AX foci forming at DSBs within the particle track are large and encompass multiple smaller and closely localised foci, which we designate as clustered gH2AX foci. These foci are repaired with slow kinetics by DNA non-homologous end-joining (NHEJ) in G1 phase with the magnitude of complexity diminishing with time. These clustered foci (containing 10 or more individual foci) represent a signature of DSBs caused by high LET heavy particle radiation. We also identified simple gH2AX foci distant from the track, which resemble those arising after X-ray exposure, which we attribute to low LET delta-electron induced DSBs. They are rapidly repaired by NHEJ. Clustered gH2AX foci induced by heavy particle radiation cause prolonged checkpoint arrest compared to simple gH2AX foci following X-irradiation. However, mitotic entry was observed when ~10 clustered foci remain. Thus, cells can progress into mitosis with multiple clusters of DSBs following the traversal of a heavy particle. | |||||
| 書誌情報 |
PLoS ONE (Online only:URL:http://www.plosone.org) 巻 8, 号 8, p. e70107, 発行日 2013-08 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1932-6203 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pone.0070107 | |||||
