WEKO3
アイテム
Hypoxia-Induced Cerebral Angiogenesis in Mouse Cortex with Two-Photon Microscopy
https://repo.qst.go.jp/records/46909
https://repo.qst.go.jp/records/46909611b9942-48d2-4937-bdfd-8f78cf1e45cf
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-11-07 | |||||
| タイトル | ||||||
| タイトル | Hypoxia-Induced Cerebral Angiogenesis in Mouse Cortex with Two-Photon Microscopy | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Masamoto, Kazuto
× Masamoto, Kazuto× Takuwa, Hiroyuki× Tomita, Yutaka× Toriumi, Haruki× Unekawa, Miyuki× Taniguchi, Jyunko× Kawaguchi, Hiroshi× Itoh, Yoshiaki× Suzuki, Norihiro× Ito, Hiroshi× Kanno, Iwao× 正本 和人× 田桑 弘之× 冨田 裕× 鳥海 春樹× 畝川 美悠紀× 谷口 順子× 川口 拓之× 伊藤 浩× 菅野 巖 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 µm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB. | |||||
| 書誌情報 |
Oxygen Transport to Tissue XXXV (Advances in Experimental Medicine and Biology ; v.789) 巻 789, p. 15-20, 発行日 2013-07 |
|||||
| 出版者 | ||||||
| 出版者 | Springer | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0065-2598 | |||||
