@article{oai:repo.qst.go.jp:00046909,
 author = {Masamoto, Kazuto and Takuwa, Hiroyuki and Tomita, Yutaka and Toriumi, Haruki and Unekawa, Miyuki and Taniguchi, Jyunko and Kawaguchi, Hiroshi and Itoh, Yoshiaki and Suzuki, Norihiro and Ito, Hiroshi and Kanno, Iwao and 正本 和人 and 田桑 弘之 and 冨田 裕 and 鳥海 春樹 and 畝川 美悠紀 and 谷口 順子 and 川口 拓之 and 伊藤 浩 and 菅野 巖},
 journal = {Oxygen Transport to Tissue XXXV (Advances in Experimental Medicine and Biology ; v.789)},
 month = {Jul},
 note = {To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 µm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.},
 pages = {15--20},
 title = {Hypoxia-Induced Cerebral Angiogenesis in Mouse Cortex with Two-Photon Microscopy},
 volume = {789},
 year = {2013}
}