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Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo.
https://repo.qst.go.jp/records/46732
https://repo.qst.go.jp/records/46732be28bcd2-cb96-4bfa-a7b2-65cd2dc77876
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-05 | |||||
| タイトル | ||||||
| タイトル | Manganese-Enhanced MRI Reveals Early-Phase Radiation-Induced Cell Alterations In Vivo. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Saito, Shigeyoshi
× Saito, Shigeyoshi× Hasegawa, Sumitaka× Sekita, Aiko× Bakalova-Zheleva, Rumiana× Furukawa, Takako× Murase, Kenya× Saga, Tsuneo× Aoki, Ichio× 齋藤 茂芳× 長谷川 純崇× バカロバ ルミアナ× 古川 高子× 村瀬 研也× 佐賀 恒夫× 青木 伊知男 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For tumor radiotherapy, the in vivo detection of early cellular responses is important for predicting therapeutic efficacy. Mn2+ is used as a positive contrast agent in manganese-enhanced MRI (MEMRI) and is expected to behave as a mimic of Ca2+ in many biologic systems. We conducted in vitro and in vivo MRI experiments with Mn2+ to investigate whether MEMRI can be used to detect cell alterations as an early-phase tumor response after radiotherapy. Colon-26 cells or a subcutaneously grafted colon-26 tumor model were irradiated with 20 Gy of X-rays. One day after irradiation, a significant augmentation of G2–M-phase cells, indicating a cell-cycle arrest, was observed in the irradiated cells in comparison with the control cells, although both early and late apoptotic alterations were rarely observed. The MEMRI signal in radiation-exposed tumor cells (R1: 0.77 +- 0.01 s−1) was significantly lower than that in control cells (R1: 0.82 +- 0.01 s−1) in vitro. MEMRI signal reduction was also observed in the in vivo tumor model 24 hours after irradiation (R1 of radiation: 0.97 +- 0.02 s−1, control: 1.10 +- 0.02 s−1), along with cell-cycle and proliferation alterations identified with immunostaining (cyclin D1 and Ki-67). Therefore, MEMRI after tumor radiotherapy was successfully used to detect cell alterations as an early-phase cellular response in vitro and in vivo. | |||||
| 書誌情報 |
Cancer Research 巻 73, 号 11, p. 3216-3224, 発行日 2013-05 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0008-5472 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1158/0008-5472.can-12-3837 | |||||
