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Method for Assessing X-ray-Induced Hydroxyl Radical Scavenging Activity of Biological Compounds/Materials
https://repo.qst.go.jp/records/46688
https://repo.qst.go.jp/records/466889d16454a-d826-4d72-a206-eac41b55aebd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Ueno.M.2013.J.Clin.Biochem.Nutr.52.95-100.pdf (537.2 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2013-05-15 | |||||
| タイトル | ||||||
| タイトル | Method for Assessing X-ray-Induced Hydroxyl Radical Scavenging Activity of Biological Compounds/Materials | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ueno, Megumi
× Ueno, Megumi× Nakanishi, Ikuo× Ken-ichiro, Matsumoto× Megumi, Ueno× Ikuo, Nakanishi× Kenichiro, Matsumoto |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A method for correctly assessing hydroxyl radical scavenging activity of antioxidative chemicals and/or biological compounds/materials was proposed. This method can simultaneously assess two factors, i.e. hydroxyl radical scavenging and 5,5-dimethyl-2-hydroxy-1-pyrrolidine-N-oxide (hydroxyl radical adduct of 5,5-dimethyl-1-pyrroline-N-oxide) reducing ability, as antioxidative properties. In this paper, some biologically common hydrophilic molecules, cell culture media, and rat plasma were tested. X-ray induced hydroxyl radical can be detected using the electron paramagnetic resonance spin trapping technique. Using X-ray irradiation of the reaction mixture as the hydroxyl radical source, the true hydroxyl radical scavenging ability of the subjected antioxidant can be assessed. In addition, the method simultaneously measures the reduction of 5,5-dimethyl-2-hydroxy-1-pyrrolidine-N-oxide, to estimate the reducing ability of the antioxidant. Biological materials, such as sugars and proteins, could abolish hydroxyl radical at the biological concentration. Ascorbic acid showed reducing ability at the biological concentration. The simultaneous assessment of hydroxyl radical scavenging and reducing ability of antioxidants can be an informative index for antioxidants. | |||||
| 書誌情報 |
Journal of Clinical Biochemistry and Nutrition 巻 52, 号 2, p. 95-100, 発行日 2013-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0912-0009 | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 23525190 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3164/jcbn.12-75 | |||||
| 関連サイト | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.3164/jcbn.12-75 | |||||
