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A modified protocol for accurate detection of cell fusion-mediated premature chromosome condensation in human peripheral blood lymphocytes
https://repo.qst.go.jp/records/46571
https://repo.qst.go.jp/records/46571e0deba09-1668-4666-af5b-ec7a5711c1b6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-07-02 | |||||
| タイトル | ||||||
| タイトル | A modified protocol for accurate detection of cell fusion-mediated premature chromosome condensation in human peripheral blood lymphocytes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suto, Yumiko
× Suto, Yumiko× Akiyma, Miho× Gotoh, Takaya× Hirai, Momoki× 數藤 由美子× 穐山 美穂× 後藤 孝也× 平井 百樹 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | When interphase cells are fused with mitotic cells, loosely distributing chromatins in nuclei are induced to form prematurely condensed chromosomes. In this paper we report a modified protocol to unequivocally detect prematurely condensed chromosomes in human peripheral blood lymphocytes that were fused with mitotic Chinese hamster ovary (CHO) cells. To examine cell fusion-mediated premature chromosome condensation (PCC), we conducted morphological analysis by differential interference contrast microscopy and molecular cytogenetic analysis by fluorescence in situ hybridization using pan-centromeric and telomeric peptide nucleic acid (PNA) probes. These modified procedures may serve to improve the usefulness of the technique of PCC in cytogenetic investigations. | |||||
| 書誌情報 |
Cytologia : International Journal of Cytology 巻 78, 号 1, p. 97-103, 発行日 2013-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0011-4545 | |||||
