@article{oai:repo.qst.go.jp:00046571,
 author = {Suto, Yumiko and Akiyma, Miho and Gotoh, Takaya and Hirai, Momoki and 數藤 由美子 and 穐山 美穂 and 後藤 孝也 and 平井 百樹},
 issue = {1},
 journal = {Cytologia : International Journal of Cytology},
 month = {Jul},
 note = {When interphase cells are fused with mitotic cells, loosely distributing chromatins in nuclei are induced to form prematurely condensed chromosomes.  In this paper we report a modified protocol to unequivocally detect prematurely condensed chromosomes in human peripheral blood lymphocytes that were fused with mitotic Chinese hamster ovary (CHO) cells.  To examine cell fusion-mediated premature chromosome condensation (PCC), we conducted morphological analysis by differential interference contrast microscopy and molecular cytogenetic analysis by fluorescence in situ hybridization using pan-centromeric and telomeric peptide nucleic acid (PNA) probes.  These modified procedures may serve to improve the usefulness of the technique of PCC in cytogenetic investigations.},
 pages = {97--103},
 title = {A modified protocol for accurate detection of cell fusion-mediated premature chromosome condensation in human peripheral blood lymphocytes},
 volume = {78},
 year = {2013}
}