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Functional analysis of lysosome during mouse preimplantation
https://repo.qst.go.jp/records/46547
https://repo.qst.go.jp/records/465476549317a-9317-4ed5-9374-4d3383c907c0
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-05-08 | |||||
| タイトル | ||||||
| タイトル | Functional analysis of lysosome during mouse preimplantation | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tsukamoto, Satoshi
× Tsukamoto, Satoshi× Hara, Taichi× Yamamoto, Atsushi× Ohta, Yuki× Wada, Ayako× Ishida, Yuka× Kito, Seiji× Nishikawa, Tetsu× Minami, Naojiro× Sato, Ken× Kokubo, Toshiaki× 塚本 智史× 山本 篤× 和田 彩子× 石田 有香× 鬼頭 靖司× 西川 哲× 南 直治郎× 小久保 年章 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers decreased slightly in blastocysts. Consistently, the protein expression level of mature cathepsins B and D was high from the one-cell to morula stages but low in the blastocyst stage. One-cell embryos injected with siRNAs targeted to both lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) were developmentally arrested at the two-cell stage. Pharmacological inhibition of lysosomes also caused developmental retardation, resulting in accumulation of lipofuscin. Our findings highlight the functional changes in lysosomes in mouse preimplantation embryos. | |||||
| 書誌情報 |
The Journal of Reproduction and Development 巻 59, 号 1, p. 33-39, 発行日 2013-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0916-8818 | |||||
