@article{oai:repo.qst.go.jp:00046547,
 author = {Tsukamoto, Satoshi and Hara, Taichi and Yamamoto, Atsushi and Ohta, Yuki and Wada, Ayako and Ishida, Yuka and Kito, Seiji and Nishikawa, Tetsu and Minami, Naojiro and Sato, Ken and Kokubo, Toshiaki and 塚本 智史 and 山本 篤 and 和田 彩子 and 石田 有香 and 鬼頭 靖司 and 西川 哲 and 南 直治郎 and 小久保 年章},
 issue = {1},
 journal = {The Journal of Reproduction and Development},
 month = {Feb},
 note = {Lysosomes are acidic and highly dynamic organelles that are essential for macromolecule degradation and many other cellular functions. However, little is known about lysosomal function during early embryogenesis. Here, we found that the number of lysosomes increased after fertilization. Lysosomes were abundant during mouse preimplantation development until the morula stage, but their numbers decreased slightly in blastocysts. Consistently, the protein expression level of mature cathepsins B and D was high from the one-cell to morula stages but low in the blastocyst stage. One-cell embryos injected with siRNAs targeted to both lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) were developmentally arrested at the two-cell stage. Pharmacological inhibition of lysosomes also caused developmental retardation, resulting in accumulation of lipofuscin. Our findings highlight the functional changes in lysosomes in mouse preimplantation embryos.},
 pages = {33--39},
 title = {Functional analysis of lysosome during mouse preimplantation},
 volume = {59},
 year = {2013}
}