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Progesterone stimulates proliferation of a long-lived epithelial cell population in rat mammary gland
https://repo.qst.go.jp/records/46436
https://repo.qst.go.jp/records/464366806d01a-1865-4f6a-885f-585afb54a62d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-11-20 | |||||
| タイトル | ||||||
| タイトル | Progesterone stimulates proliferation of a long-lived epithelial cell population in rat mammary gland | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Imaoka, Tatsuhiko
× Imaoka, Tatsuhiko× Hisatune, Hiromi× Sakanishi, Yoshimi× Nishimura, Yukiko× Nishimura, Mayumi× Shimada, Yoshiya× 今岡 達彦× 久恒 裕美× 坂西 良彌× 西村 由希子× 西村 まゆみ× 島田 義也 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Long-lived somatic cells such as stem/progenitor cells may progressively accumulate oncogenic mutations and cause cancer. Some evidence suggests that premenopausal administration of progesterone confers a long-term increased risk of breast cancer. Aim: To clarify the effect of progesterone on long-lived mammary epithelial cells in rats. Materials and Methods: Female Sprague-Dawley rats (3 and 7 weeks of age) were implanted subcutaneously with 14-day slow-release pellets of 5-bromo-2'-deoxyuridine [BrdU] and were sacrificed every 2 weeks between 0 and 10 weeks after the release period. Some rats at 7 weeks of age were also implanted with progesterone and sacrificed 0 or 10 weeks after the release period. Mammary glands were examined by immunohistochemistry and immunofluorescence for BrdU, proliferative cell nuclear antigen [PCNA] and progesterone receptor [PR]. Results: After BrdU labeling of 3- and 7-week-old rats, the BrdU index decreased gradually over 10 weeks and resulted in small fractions (1-3%) of label-retaining epithelial cells [LRECs] 10 weeks after BrdU labeling in both mammary lobules and ducts. Treatment with progesterone during labeling significantly increased the fraction of long-lived LRECs in lobules and ducts by 9- and 4-fold, respectively. The long-lived LREC population in the ducts was enriched for PCNA- and PR-positive cells, but the percentage of positive cells was not affected by progesterone in either lobules or ducts. Conclusions: Progesterone stimulates proliferation of a long-lived epithelial cell population in the mammary lobules and ducts of rats. Such cells in the duct are characterized by a high proliferation rate and PR expression. |
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| 書誌情報 |
Journal of Endocrinological Investigation 巻 35, 号 9, p. 828-834, 発行日 2012-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0391-4097 | |||||
