{"created":"2023-05-15T14:36:04.654816+00:00","id":46436,"links":{},"metadata":{"_buckets":{"deposit":"2e4283e8-2239-4e74-b780-31cae0ce24b3"},"_deposit":{"created_by":1,"id":"46436","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46436"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046436","sets":["1"]},"author_link":["462588","462581","462585","462582","462586","462580","462577","462579","462578","462587","462583","462584"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9","bibliographicPageEnd":"834","bibliographicPageStart":"828","bibliographicVolumeNumber":"35","bibliographic_titles":[{"bibliographic_title":"Journal of Endocrinological Investigation"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Long-lived somatic cells such as stem/progenitor cells may progressively accumulate oncogenic mutations and cause cancer. Some evidence suggests that premenopausal administration of progesterone confers a long-term increased risk of breast cancer. \nAim: To clarify the effect of progesterone on long-lived mammary epithelial cells in rats. \nMaterials and Methods: Female Sprague-Dawley rats (3 and 7 weeks of age) were implanted subcutaneously with 14-day slow-release pellets of 5-bromo-2'-deoxyuridine [BrdU] and were sacrificed every 2 weeks between 0 and 10 weeks after the release period. Some rats at 7 weeks of age were also implanted with progesterone and sacrificed 0 or 10 weeks after the release period. Mammary glands were examined by immunohistochemistry and immunofluorescence for BrdU, proliferative cell nuclear antigen [PCNA] and progesterone receptor [PR]. \nResults: After BrdU labeling of 3- and 7-week-old rats, the BrdU index decreased gradually over 10 weeks and resulted in small fractions (1-3%) of label-retaining epithelial cells [LRECs] 10 weeks after BrdU labeling in both mammary lobules and ducts. Treatment with progesterone during labeling significantly increased the fraction of long-lived LRECs in lobules and ducts by 9- and 4-fold, respectively. The long-lived LREC population in the ducts was enriched for PCNA- and PR-positive cells, but the percentage of positive cells was not affected by progesterone in either lobules or ducts. \nConclusions: Progesterone stimulates proliferation of a long-lived epithelial cell population in the mammary lobules and ducts of rats. Such cells in the duct are characterized by a high proliferation rate and PR expression.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0391-4097","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Imaoka, Tatsuhiko"}],"nameIdentifiers":[{"nameIdentifier":"462577","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hisatune, Hiromi"}],"nameIdentifiers":[{"nameIdentifier":"462578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sakanishi, Yoshimi"}],"nameIdentifiers":[{"nameIdentifier":"462579","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimura, Yukiko"}],"nameIdentifiers":[{"nameIdentifier":"462580","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishimura, Mayumi"}],"nameIdentifiers":[{"nameIdentifier":"462581","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Yoshiya"}],"nameIdentifiers":[{"nameIdentifier":"462582","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"今岡 達彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462583","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"久恒  裕美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462584","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"坂西 良彌","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462585","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西村 由希子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462586","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"西村 まゆみ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462587","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"島田 義也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462588","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Progesterone stimulates proliferation of a long-lived epithelial cell population in rat mammary gland","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Progesterone stimulates proliferation of a long-lived epithelial cell population in rat mammary gland"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-20"},"publish_date":"2012-11-20","publish_status":"0","recid":"46436","relation_version_is_last":true,"title":["Progesterone stimulates proliferation of a long-lived epithelial cell population in rat mammary gland"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:51:49.849110+00:00"}