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Reduced Proliferative and Differentiative Activity of Mouse Pink-eyed Dilution Melanoblasts is Related to Apoptosis
https://repo.qst.go.jp/records/46433
https://repo.qst.go.jp/records/4643368de39ac-0db3-4675-b356-92c4bd8a01ae
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-11-09 | |||||
| タイトル | ||||||
| タイトル | Reduced Proliferative and Differentiative Activity of Mouse Pink-eyed Dilution Melanoblasts is Related to Apoptosis | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirobe, Tomohisa
× Hirobe, Tomohisa× Terunuma, Emi× 廣部 知久× 照沼 絵美 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The mouse pink-eyed dilution (p) locus is known to control the melanin content, melanosome morphology and tyrosinase activity in melanocytes. However, it is not well clarified whether the p allele is involved in regulating melanocyte proliferation, differentiation and death. The aim of this study is to investigate in detail the role of the p allele in melanocyte proliferation, differentiation and death using cell culture system. The epidermal cell suspensions of the neonatal dorsal skin derived from wild type mice at the p locus (black, C57BL/10JHir-P/P) and their congenic mutant (pink-eyed dilution, C57BL/10JHir-p/p) were cultured with serum-free melanoblast-proliferation medium (MDMDF) and melanocyte-proliferation medium (MDMD). The proliferation and differentiation of p/p melanoblasts in MDMDF or MDMD were greatly inhibited compared with those of P/P melanoblasts and melanocytes. It is possible that apoptosis is related to the reduced proliferative and differentiative activity of p/p melanoblasts/melanocytes. The addition of apoptosis-inhibitors, such as caspase-9 inhibitor (C9I) and Bax-inhibiting peptide (BIP) into MDMDF or MDMD stimulated the proliferation and differentiation of p/p melanoblasts. On the contrary, in P/P melanoblasts and melanocytes, C9I and BIP failed to stimulate their proliferation and differentiation. The number of apoptotic keratinocytes and melanoblasts/melanocytes in p/p mice was greater than in P/P mice. Moreover, expression of C9 and Bax in keratinocytes and melanoblasts/melanocytes in p/p mice was greater than in P/P mice. These results suggest that the increased apoptosis in keratinocytes and melanoblasts/melanocytes is related to the reduced proliferative and differentiative activity of p/p melanoblasts. | |||||
| 書誌情報 |
Zoological Science 巻 29, 号 11, p. 725-732, 発行日 2012-11 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0289-0003 | |||||
