{"created":"2023-05-15T14:36:04.526237+00:00","id":46433,"links":{},"metadata":{"_buckets":{"deposit":"96fe0792-d494-46a4-b579-73ce777d8285"},"_deposit":{"created_by":1,"id":"46433","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46433"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046433","sets":["1"]},"author_link":["462545","462546","462544","462547"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"732","bibliographicPageStart":"725","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"Zoological Science"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The mouse pink-eyed dilution (p) locus is known to control the melanin content, melanosome morphology and tyrosinase activity in melanocytes. However, it is not well clarified whether the p allele is involved in regulating melanocyte proliferation, differentiation and death. The aim of this study is to investigate in detail the role of the p allele in melanocyte proliferation, differentiation and death using cell culture system. The epidermal cell suspensions of the neonatal dorsal skin derived from wild type mice at the p locus (black, C57BL/10JHir-P/P) and their congenic mutant (pink-eyed dilution, C57BL/10JHir-p/p) were cultured with serum-free melanoblast-proliferation medium (MDMDF) and melanocyte-proliferation medium (MDMD). The proliferation and differentiation of p/p melanoblasts in MDMDF or MDMD were greatly inhibited compared with those of P/P melanoblasts and melanocytes. It is possible that apoptosis is related to the reduced proliferative and differentiative activity of p/p melanoblasts/melanocytes. The addition of apoptosis-inhibitors, such as caspase-9 inhibitor (C9I) and Bax-inhibiting peptide (BIP) into MDMDF or MDMD stimulated the proliferation and differentiation of p/p melanoblasts. On the contrary, in P/P melanoblasts and melanocytes, C9I and BIP failed to stimulate their proliferation and differentiation. The number of apoptotic keratinocytes and melanoblasts/melanocytes in p/p mice was greater than in P/P mice. Moreover, expression of C9 and Bax in keratinocytes and melanoblasts/melanocytes in p/p mice was greater than in P/P mice. These results suggest that the increased apoptosis in keratinocytes and melanoblasts/melanocytes is related to the reduced proliferative and differentiative activity of p/p melanoblasts.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0289-0003","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"462544","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Terunuma, Emi"}],"nameIdentifiers":[{"nameIdentifier":"462545","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462546","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"照沼 絵美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"462547","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Reduced Proliferative and Differentiative Activity of Mouse Pink-eyed Dilution Melanoblasts is Related to Apoptosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reduced Proliferative and Differentiative Activity of Mouse Pink-eyed Dilution Melanoblasts is Related to Apoptosis"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-11-09"},"publish_date":"2012-11-09","publish_status":"0","recid":"46433","relation_version_is_last":true,"title":["Reduced Proliferative and Differentiative Activity of Mouse Pink-eyed Dilution Melanoblasts is Related to Apoptosis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:51:51.942804+00:00"}