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Long-term effects of hepatocyte growth factor gene therapy in rat myocardial infarct model
https://repo.qst.go.jp/records/46378
https://repo.qst.go.jp/records/463785604fc93-148a-4507-85ca-c63d99314b59
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2012-08-14 | |||||
| タイトル | ||||||
| タイトル | Long-term effects of hepatocyte growth factor gene therapy in rat myocardial infarct model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Nan, Jin Yong
× Nan, Jin Yong× Inubushi, Masayuki× Masamoto, Kazuto× Odaka, Kenichi× Aoki, Ichio× Tsuji, Atsushi× Sagara, Masashi× Koizumi, Mitsuru× Saga, Tsuneo× 金 永男× 犬伏 正幸× 正本 和人× 小高 謙一× 青木 伊知男× 辻 厚至× 相良 雅史× 小泉 満× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the long-term effects of human hepatocyte growth factor (HGF) gene therapy in a rat myocardial infarct model. Treatment adenovirus coexpressing the HGF therapeutic gene and the human sodium/iodide symporter (NIS) reporter gene or control adenovirus expressing the NIS gene alone were injected directly into the infarct border zone immediately after permanent coronary ligation in rats (n=6 each). A uniform disease state was confirmed in the acute phase in terms of impaired left ventricular (LV) function by cine magnetic resonance imaging (MRI), large infarct extent by (99m)Tc-tetrofosmin single-photon emission computed tomography (SPECT) and successful gene transfer and expression by (99m)TcO(4)(-) SPECT. After a 10-week follow-up, repeated cine MRI demonstrated no significant difference in the LV ejection fraction between the time points or groups, but a significantly increased end-diastolic volume from the acute to the chronic phase without a significant difference between the groups. Capillary density was significantly higher in the treatment group, whereas arteriole density remained unchanged. Two-photon excitation fluorescence microscopy revealed extremely thin capillaries (2-5 um), and their irregular networks increased in the infarct border zone of the treated myocardium. Our results indicated that single HGF gene therapy alone induced an immature and irregular microvasculature. | |||||
| 書誌情報 |
Gene Therapy 巻 19, 号 8, p. 836-843, 発行日 2011-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-7128 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1038/gt.2011.128 | |||||
