@article{oai:repo.qst.go.jp:00046378,
 author = {Nan, Jin Yong and Inubushi, Masayuki and Masamoto, Kazuto and Odaka, Kenichi and Aoki, Ichio and Tsuji, Atsushi and Sagara, Masashi and Koizumi, Mitsuru and Saga, Tsuneo and 金 永男 and 犬伏 正幸 and 正本 和人 and 小高 謙一 and 青木 伊知男 and 辻 厚至 and 相良 雅史 and 小泉 満 and 佐賀 恒夫},
 issue = {8},
 journal = {Gene Therapy},
 month = {Sep},
 note = {We investigated the long-term effects of human hepatocyte growth factor (HGF) gene therapy in a rat myocardial infarct model. Treatment adenovirus coexpressing the HGF therapeutic gene and the human sodium/iodide symporter (NIS) reporter gene or control adenovirus expressing the NIS gene alone were injected directly into the infarct border zone immediately after permanent coronary ligation in rats (n=6 each). A uniform disease state was confirmed in the acute phase in terms of impaired left ventricular (LV) function by cine magnetic resonance imaging (MRI), large infarct extent by (99m)Tc-tetrofosmin single-photon emission computed tomography (SPECT) and successful gene transfer and expression by (99m)TcO(4)(-) SPECT. After a 10-week follow-up, repeated cine MRI demonstrated no significant difference in the LV ejection fraction between the time points or groups, but a significantly increased end-diastolic volume from the acute to the chronic phase without a significant difference between the groups. Capillary density was significantly higher in the treatment group, whereas arteriole density remained unchanged. Two-photon excitation fluorescence microscopy revealed extremely thin capillaries (2-5 um), and their irregular networks increased in the infarct border zone of the treated myocardium. Our results indicated that single HGF gene therapy alone induced an immature and irregular microvasculature.},
 pages = {836--843},
 title = {Long-term effects of hepatocyte growth factor gene therapy in rat myocardial infarct model},
 volume = {19},
 year = {2011}
}