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Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions of CD133+highly tumorigenic cells in mouse colon carcinoma
https://repo.qst.go.jp/records/46232
https://repo.qst.go.jp/records/4623205b54a74-8d18-4fc7-9cec-67e2cbc51430
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-12-21 | |||||
| タイトル | ||||||
| タイトル | Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions of CD133+highly tumorigenic cells in mouse colon carcinoma | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yoshii, Yukie
× Yoshii, Yukie× Furukawa, Takako× Kiyono, Yasushi× Watanabe, Ryo× Waki, Atsuo× Mori, Tetsuya× Yoshii, Hiroshi× Oh, Myungmi× Asai, Tatsuya× Okazawa, Hidehiko× Fujibayashi, Yasuhisa× et.al× 吉井 幸恵× 古川 高子× 清野 泰 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) is a potential imaging agent of hypoxic tumor for use with PET. Recent literature indicates that cancer cells expressing CD133, which is a frequently used marker for so-called cancer stem cells or cancer stem cell-like cells (collectively referred here as CSC's), contribute to tumor's therapeutic resistance and metastasis ability and possess hypoxia resistance. Here, we investigated relationships between 64Cu- ATSM accumulation and existence of CD133+ cells using mouse colon carcinoma (Colon-26) tumor. Methods: Intratumor distribution of 64Cu-ATSM and 18F-fluorodeoxyglucose (18FDG) was compared with immunohistochemical staining for CD133 with a Colon-26 model. In addition, in vitro characterization of CD133+ Colon-26 cells was performed. Results: In Colon-26 tumors, 64Cu-ATSM localized preferentially in regions with a high density of CD133+ cells. The percentage of CD133+ cells was 11-fold higher in 64Cu-ATSM high uptake regions compared with 18FDG high (but 64Cu-ATSM low) uptake regions. CD133+ Colon-26 cells showed characteristics, which have been previously linked with CSC's in other cancer cell lines, such as high colony-forming ability, high tumor-initiating ability, and hypoxia resistance. In vitro culturing of Colon- 26 cells under hypoxia and/or glucose starvation showed that CD133+ cells were resistant to hypoxia and glucose starvation, and 64Cu-ATSM uptake was increased under such conditions. Conclusions: Our findings indicate that, in Colon-26 tumors, 64Cu-ATSM accumulates in CD133+ cell-rich regions and that CD133+ Colon-26 cells were highly tumorigenic and resistant to hypoxia and glucose starvation. Therefore 64Cu-ATSM could be a potential imaging agent for rich regions of CD133+ cells, associated with CSC's, within tumors. |
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| 書誌情報 |
Nuclear Medicine and Biology 巻 37, 号 4, p. 395-404, 発行日 2010-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-8051 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.nucmedbio.2009.12.011 | |||||
