{"created":"2023-05-15T14:35:55.714580+00:00","id":46232,"links":{},"metadata":{"_buckets":{"deposit":"ec2ccf1f-4ed8-4555-bfea-b1cfb7eb333a"},"_deposit":{"created_by":1,"id":"46232","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46232"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046232","sets":["1"]},"author_link":["460391","460392","460390","460389","460395","460394","460397","460396","460401","460402","460398","460400","460403","460393","460399"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"404","bibliographicPageStart":"395","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: 64Cu-diacetyl-bis (N4-methylthiosemicarbazone) (64Cu-ATSM) is a potential imaging agent of hypoxic tumor for use with PET. Recent literature indicates that cancer cells expressing CD133, which is a frequently used marker for so-called cancer stem cells or cancer stem cell-like cells (collectively referred here as CSC's), contribute to tumor's therapeutic resistance and metastasis ability and possess hypoxia resistance. Here, we investigated relationships between 64Cu-\nATSM accumulation and existence of CD133+ cells using mouse colon carcinoma (Colon-26) tumor.  \nMethods: Intratumor distribution of 64Cu-ATSM and 18F-fluorodeoxyglucose (18FDG) was compared with immunohistochemical staining for CD133 with a Colon-26 model. In addition, in vitro characterization of CD133+ Colon-26 cells was performed.  \nResults: In Colon-26 tumors, 64Cu-ATSM localized preferentially in regions with a high density of CD133+ cells. The percentage of CD133+ cells was 11-fold higher in 64Cu-ATSM high uptake regions compared with 18FDG high (but 64Cu-ATSM low) uptake regions. CD133+ Colon-26 cells showed characteristics, which have been previously linked with CSC's in other cancer cell lines, such as high colony-forming ability, high tumor-initiating ability, and hypoxia resistance. In vitro culturing of Colon-\n26 cells under hypoxia and/or glucose starvation showed that CD133+ cells were resistant to hypoxia and glucose starvation, and 64Cu-ATSM uptake was increased under such conditions.  \nConclusions: Our findings indicate that, in Colon-26 tumors, 64Cu-ATSM accumulates in CD133+ cell-rich regions and that CD133+ Colon-26 cells were highly tumorigenic and resistant to hypoxia and glucose starvation. Therefore 64Cu-ATSM could be a potential imaging agent for rich regions of CD133+ cells, associated with CSC's, within tumors.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.nucmedbio.2009.12.011","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yoshii, Yukie"}],"nameIdentifiers":[{"nameIdentifier":"460389","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Furukawa, Takako"}],"nameIdentifiers":[{"nameIdentifier":"460390","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kiyono, Yasushi"}],"nameIdentifiers":[{"nameIdentifier":"460391","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Ryo"}],"nameIdentifiers":[{"nameIdentifier":"460392","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Waki, Atsuo"}],"nameIdentifiers":[{"nameIdentifier":"460393","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mori, Tetsuya"}],"nameIdentifiers":[{"nameIdentifier":"460394","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshii, Hiroshi"}],"nameIdentifiers":[{"nameIdentifier":"460395","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oh, Myungmi"}],"nameIdentifiers":[{"nameIdentifier":"460396","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Asai, Tatsuya"}],"nameIdentifiers":[{"nameIdentifier":"460397","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazawa, Hidehiko"}],"nameIdentifiers":[{"nameIdentifier":"460398","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujibayashi, Yasuhisa"}],"nameIdentifiers":[{"nameIdentifier":"460399","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"460400","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉井 幸恵","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460401","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"古川 高子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460402","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"清野 泰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"460403","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions  of CD133+highly tumorigenic cells in mouse colon carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions  of CD133+highly tumorigenic cells in mouse colon carcinoma"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-12-21"},"publish_date":"2011-12-21","publish_status":"0","recid":"46232","relation_version_is_last":true,"title":["Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions  of CD133+highly tumorigenic cells in mouse colon carcinoma"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:54:18.270428+00:00"}