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In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs
https://repo.qst.go.jp/records/46185
https://repo.qst.go.jp/records/461853a89096f-101d-46b3-b1d3-7b2eb48fe82d
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-10-25 | |||||
| タイトル | ||||||
| タイトル | In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Kato, Takamitsu
× Kato, Takamitsu× Tsuda, Akihisa× Uesaka, Mitsuru× Fujimori, Akira× Kamada, Tadashi× Tsujii, Hirohiko× Okayasu, Ryuichi× 加藤 宝光× 津田 晃久× 上坂 充× 藤森 亮× 鎌田 正× 辻井 博彦× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | BACKGROUND: Chordoma, a rare cancer, is usually treated with surgery and/or radiation. However, very limited characterizations of chordoma cells are available due to a minimal availability (only two lines validated by now) and the extremely long doubling time. In order to overcome this situation, we successfully derived a cell line with a shorter doubling time from the first validated chordoma line U-CH1 and obtained invaluable cell biological data. \nMETHOD: After isolating a subpopulation of U-CH1 cells with a short doubling time (U-CH1-N), cell growth, cell cycle distribution, DNA content, chromosome number, p53 status, and cell survival were examined after exposure to X-rays, heavy ions, camptothecin, mitomycin C, cisplatin and bleocin. These data were compared with those of HeLa (cervical cancer) and U87-MG (glioblastoma) cells. \nRESULTS: The cell doubling times for HeLa, U87-MG and U-CH1-N were approximately 18 h, 24 h and 3 days respectively. Heavy ion irradiation resulted in more efficient cell killing than x-rays in all three cell lines. Relative biological effectiveness (RBE) at 10% survival for U-CH1-N was about 2.45 for 70keV/micro m carbon and 3.86 for 200keV/micro m iron ions. Of the four chemicals, bleocin showed the most marked cytotoxic effect on U-CH1-N. \nCONCLUSION: Our data provide the first comprehensive cellular characterization using cells of chordoma origin and furnish the biological basis for successful clinical results of chordoma treatment by heavy ions. |
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| 書誌情報 |
Radiation Oncology (Online only URL:http://www.ro-journal.com/) 巻 6, p. 116, 発行日 2011-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1748-717X | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.ro-journal.com/content/6/1/116 | |||||
| 関連名称 | http://www.ro-journal.com/content/6/1/116 | |||||
