{"created":"2023-05-15T14:35:53.679221+00:00","id":46185,"links":{},"metadata":{"_buckets":{"deposit":"ee1c695b-eac9-4e55-921c-56d1f9d1f108"},"_deposit":{"created_by":1,"id":"46185","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46185"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046185","sets":["1"]},"author_link":["459887","459874","459885","459875","459876","459884","459879","459878","459880","459886","459881","459877","459882","459883"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"116","bibliographicVolumeNumber":"6","bibliographic_titles":[{"bibliographic_title":"Radiation Oncology (Online only URL:http://www.ro-journal.com/)"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"BACKGROUND: Chordoma, a rare cancer, is usually treated with surgery and/or radiation. However, very limited characterizations of chordoma cells are available due to a minimal availability (only two lines validated by now) and the extremely long doubling time. In order to overcome this situation, we successfully derived a cell line with a shorter doubling time from the first validated chordoma line U-CH1 and obtained invaluable cell biological data.\n\\nMETHOD: After isolating a subpopulation of U-CH1 cells with a short doubling time (U-CH1-N), cell growth, cell cycle distribution, DNA content, chromosome number, p53 status, and cell survival were examined after exposure to X-rays, heavy ions, camptothecin, mitomycin C, cisplatin and bleocin. These data were compared with those of HeLa (cervical cancer) and U87-MG (glioblastoma) cells.\n\\nRESULTS: The cell doubling times for HeLa, U87-MG and U-CH1-N were approximately 18 h, 24 h and 3 days respectively. Heavy ion irradiation resulted in more efficient cell killing than x-rays in all three cell lines. Relative biological effectiveness (RBE) at 10% survival for U-CH1-N was about 2.45 for 70keV/micro m carbon and 3.86 for 200keV/micro m iron ions. Of the four chemicals, bleocin showed the most marked cytotoxic effect on U-CH1-N.\n\\nCONCLUSION: Our data provide the first comprehensive cellular characterization using cells of chordoma origin and furnish the biological basis for successful clinical results of chordoma treatment by heavy ions.","subitem_description_type":"Abstract"}]},"item_8_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://www.ro-journal.com/content/6/1/116"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ro-journal.com/content/6/1/116","subitem_relation_type_select":"URI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1748-717X","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kato, Takamitsu"}],"nameIdentifiers":[{"nameIdentifier":"459874","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuda, Akihisa"}],"nameIdentifiers":[{"nameIdentifier":"459875","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uesaka, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"459876","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujimori, Akira"}],"nameIdentifiers":[{"nameIdentifier":"459877","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kamada, Tadashi"}],"nameIdentifiers":[{"nameIdentifier":"459878","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsujii, Hirohiko"}],"nameIdentifiers":[{"nameIdentifier":"459879","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okayasu, Ryuichi"}],"nameIdentifiers":[{"nameIdentifier":"459880","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤 宝光","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459881","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"津田 晃久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459882","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上坂 充","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459883","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"藤森 亮","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459884","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鎌田 正","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459885","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻井 博彦","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459886","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"岡安 隆一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459887","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-10-25"},"publish_date":"2011-10-25","publish_status":"0","recid":"46185","relation_version_is_last":true,"title":["In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:54:50.471419+00:00"}