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Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts.
https://repo.qst.go.jp/records/46126
https://repo.qst.go.jp/records/4612621c491c7-01ed-4f96-96e6-57b4bedd5a18
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-07-14 | |||||
| タイトル | ||||||
| タイトル | Equivalency of nuclear transfer-derived embryonic stem cells to those derived from fertilized mouse blastocysts. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Wakayama, Sayaka
× Wakayama, Sayaka× L, Jakt Martin× Suzuki, Masako× Araki, Ryoko× Hikichi, Takafusa× Kishigami, Satoshi× Ohta, Hiroshi× Van, Thuan Nguyen× Mizutani, Eiji× Sakaide, Yuko× Senda, Sho× Tanaka, Satoshi× Okada, Mitsuhiro× Miyake, Masashi× Abe, Masumi× Nishikawa, Shinichi× Shiota, Kunio× Wakayama, Teruhiko× 荒木 良子× 安倍 真澄× 西川 伸一× 若山 照彦 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Therapeutic cloning, whereby nuclear transfer (NT) is used to generate embryonic stem cells (ESCs) from blastocysts, has been demonstrated successfully in mice and cattle. However, if NT-ESCs have abnormalities, such as those associated with the offspring produced by reproductive cloning, their scientific and medical utilities might prove limited. To evaluate the characteristics of NT-ESCs, we established more than 150 NT-ESC lines from adult somatic cells of several mouse strains. Here, we show that these NT-ESCs were able to differentiate into all functional embryonic tissues in vivo. Moreover, they were identical to blastocyst-derived ESCs in terms of their expression of pluripotency markers in the presence of tissue-dependent differentially DNA methylated regions, in DNA microarray profiles, and in high-coverage gene expression profiling. Importantly, the NT procedure did not cause irreversible damage to the nuclei. These similarities of NT-ESCs and ESCs indicate that murine therapeutic cloning by somatic cell NT can provide a reliable model for preclinical stem cell research. | |||||
| 書誌情報 |
Stem Cells 巻 24, 号 9, p. 2023-2033, 発行日 2006-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1066-5099 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1634/stemcells.2005-0537 | |||||
