WEKO3
アイテム
On-chip fraction collection for multiple selected ssDNA fragments using isolated extraction channels.
https://repo.qst.go.jp/records/46124
https://repo.qst.go.jp/records/4612437103de6-4da1-4a1d-b754-59946779ff9e
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-07-14 | |||||
| タイトル | ||||||
| タイトル | On-chip fraction collection for multiple selected ssDNA fragments using isolated extraction channels. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Li, Zheyu
× Li, Zheyu× Sun, Kai× Sunayama, Misato× Matsuo, Yasutaka× Vygantas, Mizeikis× Araki, Ryoko× Ueno, Kosei× Abe, Masumi× Misawa, Hiroaki× 砂山 美里× 荒木 良子× 安倍 真澄 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | High efficiency and high-purity fraction collection is highly sought in analysis of fragments-of-interest from selective polymerase chain reaction (PCR) products generated by High Coverage Gene Expression Profiling (HiCEP) methods. Here we demonstrate a new electrophoretic chip device enabling automatic high-efficient fractionation of multiple ssDNA target fragments during a run of separation. We used thoroughly isolated extraction channels for each selected target to reduce the risk of cross-contamination between targets due to cross-talk of extraction channels. Fragments of 35, 108 and 138 b, were successfully isolated, then the recovery was PCR-amplified and assessed by capillary electrophoresis (CE) analysis. Total impurity level of the targets due to unwanted fragments of 0.7%, 2% and 6% respectively, was estimated. Difficulties in collecting multiple target factions are due to band diffusion and DNA adsorption to the walls for the fragments in the separation channel, which is generated by transferring the DNA target fraction from the extraction section to the target reservoir. Therefore, we have carefully measured band broadening and analyzed its influence on the separation resolution due to the delay. | |||||
| 書誌情報 |
Journal of Chromatography A 巻 1218, 号 7, p. 997-1003, 発行日 2010-12 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0021-9673 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.chroma.2010.12.089 | |||||
