@article{oai:repo.qst.go.jp:00046124,
 author = {Li, Zheyu and Sun, Kai and Sunayama, Misato and Matsuo, Yasutaka and Vygantas, Mizeikis and Araki, Ryoko and Ueno, Kosei and Abe, Masumi and Misawa, Hiroaki and 砂山 美里 and 荒木 良子 and 安倍 真澄},
 issue = {7},
 journal = {Journal of Chromatography A},
 month = {Dec},
 note = {High efficiency and high-purity fraction collection is highly sought in analysis of fragments-of-interest from selective polymerase chain reaction (PCR) products generated by High Coverage Gene Expression Profiling (HiCEP) methods. Here we demonstrate a new electrophoretic chip device enabling automatic high-efficient fractionation of multiple ssDNA target fragments during a run of separation. We used thoroughly isolated extraction channels for each selected target to reduce the risk of cross-contamination between targets due to cross-talk of extraction channels. Fragments of 35, 108 and 138 b, were successfully isolated, then the recovery was PCR-amplified and assessed by capillary electrophoresis (CE) analysis. Total impurity level of the targets due to unwanted fragments of 0.7%, 2% and 6% respectively, was estimated. Difficulties in collecting multiple target factions are due to band diffusion and DNA adsorption to the walls for the fragments in the separation channel, which is generated by transferring the DNA target fraction from the extraction section to the target reservoir. Therefore, we have carefully measured band broadening and analyzed its influence on the separation resolution due to the delay.},
 pages = {997--1003},
 title = {On-chip fraction collection for multiple selected ssDNA fragments using isolated extraction channels.},
 volume = {1218},
 year = {2010}
}