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High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip.
https://repo.qst.go.jp/records/46113
https://repo.qst.go.jp/records/4611373531d10-793a-4990-816c-d56556104fcf
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-06-20 | |||||
| タイトル | ||||||
| タイトル | High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Son, Kai
× Son, Kai× Suzuki, Nobuko× Li, Zheyu× Araki, Ryoko× Ueno, Kousei× Juodkazis, Saulius× Abe, Masumi× Noji, Sumihare× Misawa, Hiroaki× 鈴木 展子× 荒木 良子× 上野 貢生× 安倍 真澄 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For the fractionation of fragments of interest from selective PCR products generated by high coverage gene expression profiling (HiCEP) analysis, high-resolution with the ability to discriminate and fractionate fragments differing by one base (base pair) in size is highly required. We report here on a new 4-inch diameter spiral-channel chip device for automatic high-fidelity fractionation. Overlapping DNA fragments of 180, 181 and 182 bases, with only one-base difference in size, were successfully fractionated. The collected fragments were PCR amplified, and then evaluated by size checking analysis, DNA sequencing, and homolog search. The high-resolution fractionation has been achieved because of the combined contributions of (i) the high-resolution separation using a 30 cm long spiral channel, (ii) a blocking technique to avoid contamination from unselected fragments during CE, and (iii) precise micro-scale target extraction. Contaminations due to unselected fractions have been greatly decreased to a negligible level by optimization of the extraction position and extraction time corresponding to the targeted segment only. This technique can be adapted to a wide range of applications, such as protein or cell collections where requirements for the high purity are more important than the amount of recovered fractionated material. | |||||
| 書誌情報 |
Electrophoresis 巻 30, 号 24, p. 4277-4284, 発行日 2009-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0173-0835 | |||||
