{"created":"2023-05-15T14:35:50.527928+00:00","id":46113,"links":{},"metadata":{"_buckets":{"deposit":"55026659-c630-4642-af12-d1abd7a522a7"},"_deposit":{"created_by":1,"id":"46113","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"46113"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00046113","sets":["1"]},"author_link":["459079","459082","459088","459086","459085","459087","459089","459084","459090","459083","459080","459078","459081"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"24","bibliographicPageEnd":"4284","bibliographicPageStart":"4277","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"Electrophoresis"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For the fractionation of fragments of interest from selective PCR products generated by high coverage gene expression profiling (HiCEP) analysis, high-resolution with the ability to discriminate and fractionate fragments differing by one base (base pair) in size is highly required. We report here on a new 4-inch diameter spiral-channel chip device for automatic high-fidelity fractionation. Overlapping DNA fragments of 180, 181 and 182 bases, with only one-base difference in size, were successfully fractionated. The collected fragments were PCR amplified, and then evaluated by size checking analysis, DNA sequencing, and homolog search. The high-resolution fractionation has been achieved because of the combined contributions of (i) the high-resolution separation using a 30 cm long spiral channel, (ii) a blocking technique to avoid contamination from unselected fragments during CE, and (iii) precise micro-scale target extraction. Contaminations due to unselected fractions have been greatly decreased to a negligible level by optimization of the extraction position and extraction time corresponding to the targeted segment only. This technique can be adapted to a wide range of applications, such as protein or cell collections where requirements for the high purity are more important than the amount of recovered fractionated material.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0173-0835","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Son, Kai"}],"nameIdentifiers":[{"nameIdentifier":"459078","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Nobuko"}],"nameIdentifiers":[{"nameIdentifier":"459079","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Zheyu"}],"nameIdentifiers":[{"nameIdentifier":"459080","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Araki, Ryoko"}],"nameIdentifiers":[{"nameIdentifier":"459081","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ueno, Kousei"}],"nameIdentifiers":[{"nameIdentifier":"459082","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Juodkazis, Saulius"}],"nameIdentifiers":[{"nameIdentifier":"459083","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Abe, Masumi"}],"nameIdentifiers":[{"nameIdentifier":"459084","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Noji, Sumihare"}],"nameIdentifiers":[{"nameIdentifier":"459085","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Misawa, Hiroaki"}],"nameIdentifiers":[{"nameIdentifier":"459086","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 展子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459087","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荒木 良子","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459088","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上野 貢生","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459089","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"安倍 真澄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"459090","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip."}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-06-20"},"publish_date":"2011-06-20","publish_status":"0","recid":"46113","relation_version_is_last":true,"title":["High-fidelity fractionation of ssDNA fragments differing in size by one-base on a spiral-channel electrophoretic chip."],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:55:42.416421+00:00"}