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Unequivocal detection of radiation-induced multicentric chromosomes by fluorescence in situ hybridization using peptide nucleic acid probes
https://repo.qst.go.jp/records/46079
https://repo.qst.go.jp/records/4607996d283b1-ce0f-4429-82f9-8cacb2a43b72
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-05-24 | |||||
| タイトル | ||||||
| タイトル | Unequivocal detection of radiation-induced multicentric chromosomes by fluorescence in situ hybridization using peptide nucleic acid probes | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Suto, Yumiko
× Suto, Yumiko× Akiyma, Miho× Yamada, Yuji× 數藤 由美子× 穐山 美穂× 山田 裕司 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Multicentric chromosomes, as represented by dicentric chromosomes, are considered to be sensitive and specific biomarkers for assessing radiation dose. Efforts have been made to precisely localize centromeres by fluorescence in situ hybridization (FISH) using pan-centromeric peptide nucleic acid (PNA) probes, which bind to the target centromeric DNA sequences quickly. We analyzed radiation-induced chromosomal aberrations by our modified technique that is simpler than the standard PNA - FISH protocol. Under our experimental conditions, the detection efficiency of centromeric signals was 100%, while that of telomeric signals was 90-100%. |
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| 書誌情報 |
Cytologia : International Journal of Cytology 巻 76, 号 1, p. 1-2, 発行日 2011-03 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0011-4545 | |||||
