WEKO3
アイテム
The first life science experiments in ISS: reports of "Rad Gene"-space radiation effects on human cultured cells-
https://repo.qst.go.jp/records/45977
https://repo.qst.go.jp/records/45977786ac4d6-e52f-4c28-bfb7-70dcc69184a6
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-12-28 | |||||
| タイトル | ||||||
| タイトル | The first life science experiments in ISS: reports of "Rad Gene"-space radiation effects on human cultured cells- | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Takahashi, Akihisa
× Takahashi, Akihisa× Nagamatsu, Aiko× Suzuki, Masao× Tsuruoka, Chizuru× Omori, Katsunori× Suzuki, Hiromi× et.al× 高橋 昭久× 鈴木 雅雄× 鶴岡 千鶴 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | To clarify the biological effects of space environment, especially space radiations, a proposal of "Rad Gene" was performed as the first life science experiment with two human lymphoblastoid cell lines bearing wild-type p53 gene (wtp53) and mutated p53 gene (mp53) in an International Space Station (ISS)for 133 days. We scheduled four projects: (1) DNA damage induced by space radiations including the high linear energy transfer (LET) particles was detected as a track of gamma H2AX foci in the nuclei of these frozen cells. (2) To examine the biological effects of microgravity and space radiations on gene and protein expression of p53-dependent regulated genes, these cells were grown under microgravity and 1 gravity in ISS, and on ground for 8 days and analyzed by DNA and protein arrays. (3) p53-Dependent regulated genes were analyzed in the cultured cells after spaceflight at frozen state exposed to space radiations. (4) To clarify the effects of space radiations on the radio-adaptive response, the space flown cells at frozen state were cultured, and then exposed to challenging X-ray-irradiation. All of the radio-adaptive responses of cell killing, apoptosis, chromosomal aberrations and mutations were found only in wtp53 cells, but not in the mp53 cells. | |||||
| 書誌情報 |
Biological Sciences in Space 巻 24, 号 1, p. 17-41, 発行日 2010 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0914-9201 | |||||
