{"created":"2023-05-15T14:35:44.587648+00:00","id":45977,"links":{},"metadata":{"_buckets":{"deposit":"2c4fe4e5-cca1-4dc9-b141-897db8208c3b"},"_deposit":{"created_by":1,"id":"45977","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45977"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045977","sets":["1"]},"author_link":["457467","457470","457475","457469","457466","457471","457472","457468","457473","457474"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"41","bibliographicPageStart":"17","bibliographicVolumeNumber":"24","bibliographic_titles":[{"bibliographic_title":"Biological Sciences in Space"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To clarify the biological effects of space environment, especially space radiations, a proposal of \"Rad Gene\" was performed as the first life science experiment with two human lymphoblastoid cell lines bearing wild-type p53 gene (wtp53) and mutated p53 gene (mp53) in an International Space Station (ISS)for 133 days. We scheduled four projects: (1) DNA damage induced by space radiations including the high linear energy transfer (LET) particles was detected as a track of gamma H2AX foci in the nuclei of these frozen cells. (2) To examine the biological effects of microgravity and space radiations on gene and protein expression of p53-dependent regulated genes, these cells were grown under microgravity and 1 gravity in ISS, and on ground for 8 days and analyzed by DNA and protein arrays. (3) p53-Dependent regulated genes were analyzed in the cultured cells after spaceflight at frozen state exposed to space radiations. (4) To clarify the effects of space radiations on the radio-adaptive response, the space flown cells at frozen state were cultured, and then exposed to challenging X-ray-irradiation. All of the radio-adaptive responses of cell killing, apoptosis, chromosomal aberrations and mutations were found only in wtp53 cells, but not in the mp53 cells.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0914-9201","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Akihisa"}],"nameIdentifiers":[{"nameIdentifier":"457466","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagamatsu, Aiko"}],"nameIdentifiers":[{"nameIdentifier":"457467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Masao"}],"nameIdentifiers":[{"nameIdentifier":"457468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuruoka, Chizuru"}],"nameIdentifiers":[{"nameIdentifier":"457469","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Omori, Katsunori"}],"nameIdentifiers":[{"nameIdentifier":"457470","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Hiromi"}],"nameIdentifiers":[{"nameIdentifier":"457471","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"et.al"}],"nameIdentifiers":[{"nameIdentifier":"457472","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"高橋 昭久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"457473","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木 雅雄","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"457474","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鶴岡 千鶴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"457475","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The first life science experiments in ISS: reports of \"Rad Gene\"-space radiation effects on human cultured cells-","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The first life science experiments in ISS: reports of \"Rad Gene\"-space radiation effects on human cultured cells-"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-12-28"},"publish_date":"2010-12-28","publish_status":"0","recid":"45977","relation_version_is_last":true,"title":["The first life science experiments in ISS: reports of \"Rad Gene\"-space radiation effects on human cultured cells-"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:57:20.923402+00:00"}