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C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model
https://repo.qst.go.jp/records/45755
https://repo.qst.go.jp/records/45755da2aed80-e916-4e03-acbb-617004beb56a
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Sogawa, Chizuru
× Sogawa, Chizuru× Tsuji, Atsushi× Sudou, Hitomi× Sugyou, Aya× Yoshida, Chisato× Odaka, Kenichi× Uehara, Tomoya× Arano, Yasushi× Koizumi, Mitsuru× Saga, Tsuneo× 曽川 千鶴× 辻 厚至× 須藤 仁美× 須尭 綾× 吉田 千里× 小高 謙一× 上原 知也× 荒野 泰× 小泉 満× 佐賀 恒夫 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor arising from the gastrointestinal tract and highly expresses mutated c-kit. We aimed to develop a specific and sensitive method for detecting GISTs using radiolabeled anti-c-kit monoclonal antibody. Methods: A mutated c-kit-expressing cell clone was established by transfecting an expressing vector of mutated c-kit gene into HEK293 human embryonic kidney cells. The tumors were developed by inoculating c-kit-expressing cells into nude mice. 125I- and 111In-labeled anti-c-kit antibodies (12A8 and 41A11) were evaluated in vitro by cell binding, competitive inhibition and cellular internalization assays, and in vivo by biodistribution and imaging studies in tumor-bearing mice. Results: Both 125I- and 111In-labeled antibodies showed specific binding with c-kit-expressing cells with high affinity (dissociation constants = 2.2-7.1x109 M-1). Internalization assay showed that 125I-labeled antibodies were rapidly internalized and dehalogenated, with the release of 125I from the cells, resulting in reduction of cell-associated radioactivity with time. In contrast, 111In-labeled antibody was internalized but did not result in the reduced radioactivity associated with tumor cells. Reflecting this phenomenon, the in vivo tumor uptake of 125I-labeled antibody was low on Day 1, further decreasing with time, while tumor uptake of 111In-labeled antibody was high on Day 1, further increasing with time. The xenografted tumor was clearly visualized by scintigraphy after injection of 111In-labeled antibody. Conclusion: The anti-c-kit monoclonal antibody labeled with a metal radionuclide would be promising for c-kit-targeted imaging of GISTs. |
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| 書誌情報 |
Nuclear Medicine and Biology 巻 37, 号 2, p. 179-187, 発行日 2010-02 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0969-8051 | |||||
