{"created":"2023-05-15T14:35:34.909482+00:00","id":45755,"links":{},"metadata":{"_buckets":{"deposit":"415f16f9-56d8-444c-b573-09231f763f2a"},"_deposit":{"created_by":1,"id":"45755","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45755"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045755","sets":["1"]},"author_link":["454783","454793","454799","454797","454780","454785","454787","454791","454795","454789","454781","454786","454796","454798","454790","454792","454794","454788","454784","454782"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"187","bibliographicPageStart":"179","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"Nuclear Medicine and Biology"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Introduction: Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor arising from the gastrointestinal tract and highly expresses mutated c-kit. \nWe aimed to develop a specific and sensitive method for detecting GISTs using radiolabeled anti-c-kit monoclonal antibody.\nMethods: A mutated c-kit-expressing cell clone was established by transfecting an expressing vector of mutated c-kit gene into HEK293 human embryonic kidney cells. The tumors were developed by inoculating c-kit-expressing cells into nude mice. 125I- and 111In-labeled anti-c-kit antibodies (12A8 and 41A11) were evaluated in vitro by cell binding, competitive inhibition and cellular internalization assays, and in vivo by biodistribution and imaging studies in tumor-bearing mice.\nResults: Both 125I- and 111In-labeled antibodies showed specific binding with c-kit-expressing cells with high affinity (dissociation constants\n= 2.2-7.1x109 M-1). Internalization assay showed that 125I-labeled antibodies were rapidly internalized and dehalogenated, with the release of 125I from the cells, resulting in reduction of cell-associated radioactivity with time. In contrast, 111In-labeled antibody was internalized but did not result in the reduced radioactivity associated with tumor cells. Reflecting this phenomenon, the in vivo tumor uptake of 125I-labeled antibody was low on Day 1, further decreasing with time, while tumor uptake of 111In-labeled antibody was high on Day 1, further increasing with time. \nThe xenografted tumor was clearly visualized by scintigraphy after injection of 111In-labeled antibody.\nConclusion: The anti-c-kit monoclonal antibody labeled with a metal radionuclide would be promising for c-kit-targeted imaging of GISTs.","subitem_description_type":"Abstract"}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0969-8051","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sogawa, Chizuru"}],"nameIdentifiers":[{"nameIdentifier":"454780","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuji, Atsushi"}],"nameIdentifiers":[{"nameIdentifier":"454781","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sudou, Hitomi"}],"nameIdentifiers":[{"nameIdentifier":"454782","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugyou, Aya"}],"nameIdentifiers":[{"nameIdentifier":"454783","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshida, Chisato"}],"nameIdentifiers":[{"nameIdentifier":"454784","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Odaka, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"454785","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Uehara, Tomoya"}],"nameIdentifiers":[{"nameIdentifier":"454786","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arano, Yasushi"}],"nameIdentifiers":[{"nameIdentifier":"454787","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Koizumi, Mitsuru"}],"nameIdentifiers":[{"nameIdentifier":"454788","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Saga, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"454789","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"曽川 千鶴","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454790","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"辻 厚至","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454791","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須藤 仁美","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454792","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"須尭 綾","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454793","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"吉田 千里","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454794","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小高 謙一","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454795","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"上原 知也","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454796","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"荒野 泰","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454797","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小泉 満","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐賀 恒夫","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454799","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-02-22"},"publish_date":"2010-02-22","publish_status":"0","recid":"45755","relation_version_is_last":true,"title":["C-kit-targeted imaging of gastrointestinal stromal tumor using radiolabeled anti-c-kit monoclonal antibody in a mouse tumor model"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-15T23:59:58.604068+00:00"}