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Life cycle of human melanocytes is regulated by endothelin-1 and stem cell factor in synergy with cyclic AMP and basic fibroblast growth factor
https://repo.qst.go.jp/records/45734
https://repo.qst.go.jp/records/45734d07be64a-e4a5-4d0e-aada-dc39f02d4c25
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2010-01-27 | |||||
| タイトル | ||||||
| タイトル | Life cycle of human melanocytes is regulated by endothelin-1 and stem cell factor in synergy with cyclic AMP and basic fibroblast growth factor | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Hirobe, Tomohisa
× Hirobe, Tomohisa× Shinpo, Tsuneo× Higuchi, Kazuhiko× Sano, Tomohiko× 廣部 知久 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Although the function of human melanocytes is well characterized at cellular and molecular levels, the mechanism of the regulation of the life cycle (proliferation, differentiation, and cell death) of human melanocytes is not fully understood. Objective: This study aims to clarify what factors are involved in regulating the life cycle of human melanocytes using serum-free culture system. Methods: Human epidermal melanocytes were cultured in a serum-free growth medium supplemented with several kinds of growth factors, cytokines, and hormones and the effects of these factors on the life cycle of melanocytes were investigated in detail. Results: Of the factors tested, endothelin-1 (ET-1) stimulated the proliferation of melanoblasts and melanocytes in the presence of cyclic AMP (cAMP)-elevating factor such as dibutyryl cAMP (DBcAMP) and of basic fibroblast growth factor (bFGF). ET-1 also stimulated the proliferation and differentiation of human melanocytes in the presence of DBcAMP. Moreover, stem cell factor (SCF) stimulated the proliferation of melanoblasts and melanocytes synergistically with ET-1. The removal of ET-1 and SCF from the culture medium greatly inhibited the proliferation of melanocytes followed by apoptotic cell death. Conclusion: These results suggest that the life cycle of human melanocytes is regulated by ET-1 and SCF in synergy with cAMP and bFGF. |
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| 書誌情報 |
Journal of Dermatological Science 巻 57, 号 2, p. 123-131, 発行日 2009-12 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0923-1811 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jdermsci.2009.11.006 | |||||
