{"created":"2023-05-15T14:35:33.989992+00:00","id":45734,"links":{},"metadata":{"_buckets":{"deposit":"6b033ea3-8728-418c-8e23-80eb93ff9058"},"_deposit":{"created_by":1,"id":"45734","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"45734"},"status":"published"},"_oai":{"id":"oai:repo.qst.go.jp:00045734","sets":["1"]},"author_link":["454577","454580","454579","454581","454578"],"item_8_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"131","bibliographicPageStart":"123","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"Journal of Dermatological Science"}]}]},"item_8_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Although the function of human melanocytes is well characterized at cellular and molecular\nlevels, the mechanism of the regulation of the life cycle (proliferation, differentiation, and cell death) of\nhuman melanocytes is not fully understood.\nObjective: This study aims to clarify what factors are involved in regulating the life cycle of human\nmelanocytes using serum-free culture system.\nMethods: Human epidermal melanocytes were cultured in a serum-free growth medium supplemented\nwith several kinds of growth factors, cytokines, and hormones and the effects of these factors on the life\ncycle of melanocytes were investigated in detail.\nResults: Of the factors tested, endothelin-1 (ET-1) stimulated the proliferation of melanoblasts and\nmelanocytes in the presence of cyclic AMP (cAMP)-elevating factor such as dibutyryl cAMP (DBcAMP)\nand of basic fibroblast growth factor (bFGF). ET-1 also stimulated the proliferation and differentiation of\nhuman melanocytes in the presence of DBcAMP. Moreover, stem cell factor (SCF) stimulated the\nproliferation of melanoblasts and melanocytes synergistically with ET-1. The removal of ET-1 and SCF\nfrom the culture medium greatly inhibited the proliferation of melanocytes followed by apoptotic cell\ndeath.\nConclusion: These results suggest that the life cycle of human melanocytes is regulated by ET-1 and SCF\nin synergy with cAMP and bFGF.","subitem_description_type":"Abstract"}]},"item_8_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jdermsci.2009.11.006","subitem_relation_type_select":"DOI"}}]},"item_8_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0923-1811","subitem_source_identifier_type":"ISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hirobe, Tomohisa"}],"nameIdentifiers":[{"nameIdentifier":"454577","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shinpo, Tsuneo"}],"nameIdentifiers":[{"nameIdentifier":"454578","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Higuchi, Kazuhiko"}],"nameIdentifiers":[{"nameIdentifier":"454579","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sano, Tomohiko"}],"nameIdentifiers":[{"nameIdentifier":"454580","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"廣部 知久","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"454581","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Life cycle of human melanocytes is regulated by endothelin-1 and stem cell factor in synergy with cyclic AMP and basic fibroblast growth factor","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Life cycle of human melanocytes is regulated by endothelin-1 and stem cell factor in synergy with cyclic AMP and basic fibroblast growth factor"}]},"item_type_id":"8","owner":"1","path":["1"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-01-27"},"publish_date":"2010-01-27","publish_status":"0","recid":"45734","relation_version_is_last":true,"title":["Life cycle of human melanocytes is regulated by endothelin-1 and stem cell factor in synergy with cyclic AMP and basic fibroblast growth factor"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-05-16T00:00:14.687230+00:00"}