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The influence of mutation induction in normal human fibroblasts irradiated with X rays and iron ions
https://repo.qst.go.jp/records/45631
https://repo.qst.go.jp/records/4563140c45b29-ff12-4fa1-93b3-28b6588bff51
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-09-11 | |||||
| タイトル | ||||||
| タイトル | The influence of mutation induction in normal human fibroblasts irradiated with X rays and iron ions | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Tsuruoka, Chizuru
× Tsuruoka, Chizuru× Suzuki, Masao× Furusawa, Yoshiya× Anzai, Kazunori× Okayasu, Ryuichi× 鶴岡 千鶴× 鈴木 雅雄× 古澤 佳也× 安西 和紀× 岡安 隆一 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We investigated the influence of mutation induction on the hypoxanthine guanine phosphoribosyltransferase (hprt) locus in normal human fibroblasts irradiated with X rays or accelerated iron ions with LET ranging from 200 to 400 keV/miceometer. The relative biological effectiveness (RBE) of the mutation frequency per total number of surviving cells for iron ions with comparatively lower LET values (200 and 260 keV/miceometer) in this study was higher than 1, and the RBE for the 300 keV/miceometer, 350 keV/miceometer and 400 keV/miceometer ions was nearly 1. These results indicated that the mutation induction for iron ions decreased with increasing LET, and these of high-LET regions (over 300 keV/miceometer) and X rays were similar. The deletion spectrum of exons in hprt locus for X rays and 260 keV/miceometer-iron ions were analyzed using multiplex polymerase chain reaction (PCR). About 70% of mutant induced by iron ions showed deletion of the entire exons (total deletion), while mutant induced by X rays showed partial deletion, which one or more exons are missing, of about 70%. The results indicated that iron-ion induced mutants sustained severer damage than X-ray induced mutants even if the doseresponse curves for mutation induction were similar between X rays and iron ions. |
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| 書誌情報 |
Biological Sciences in Space 巻 23, 号 2, p. 55-60, 発行日 2009-07 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0914-9201 | |||||
