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Developmental Responses of 2-cell Embryos to Oxygen Tension and Bovine Serum Albumin in Wistar Rats
https://repo.qst.go.jp/records/45280
https://repo.qst.go.jp/records/4528018c28071-acbb-4880-b5ec-ae4421e7439c
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-10-02 | |||||
タイトル | ||||||
タイトル | Developmental Responses of 2-cell Embryos to Oxygen Tension and Bovine Serum Albumin in Wistar Rats | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
Kito, Seiji
× Kito, Seiji× Kaneko, Yumiko× Yano, Hiroko× Tateno, Shintarou× Oota, Yuki× 鬼頭 靖司× 金子 由美子× 矢野 浩子× 舘野 真太郎× 太田 有紀 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To improve rat embryo culture conditions, responses of Wistar 2-cell embryos from 2 breeders to oxygen tension (5 vs. 20%) and bovine serum albumin (BSA) (0 vs. 3 mg/ml) were examined using rat 1-cell embryo culture medium (mR1ECM). Supplementation of 3 mg/ml BSA significantly stimulated and accelerated development to the blastocyst and expanded blastocyst stages during 72 and 96 hr culture, while reduced oxygen tension stimulated cell division. Fetus development after transfer of blastocysts obtained from 72 hr culture under 5% O2 with BSA was significantly higher than those cultured under atmospheric oxygen without BSA. However, the nuclear numbers of in vitro cultured blastocysts and fetus development after embryo transfer were still significantly lower than in vivo developed blastocysts, indicating the current culture condition is still suboptimal. | |||||
書誌情報 |
Experimental Animals 巻 57, 号 2, p. 123-128, 発行日 2008-04 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1341-1357 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.jstage.jst.go.jp/browse/expanim/57/2/_contents/-char/ja/ | |||||
関連名称 | http://www.jstage.jst.go.jp/browse/expanim/57/2/_contents/-char/ja/ |