@article{oai:repo.qst.go.jp:00045280,
 author = {Kito, Seiji and Kaneko, Yumiko and Yano, Hiroko and Tateno, Shintarou and Oota, Yuki and 鬼頭 靖司 and 金子 由美子 and 矢野 浩子 and 舘野 真太郎 and 太田 有紀},
 issue = {2},
 journal = {Experimental Animals},
 month = {Apr},
 note = {To improve rat embryo culture conditions, responses of Wistar 2-cell embryos from 2 breeders to oxygen tension (5 vs. 20%) and bovine serum albumin (BSA) (0 vs. 3 mg/ml) were examined using rat 1-cell embryo culture medium (mR1ECM).  Supplementation of 3 mg/ml BSA significantly stimulated and accelerated development to the blastocyst and expanded blastocyst stages during 72 and 96 hr culture, while reduced oxygen tension stimulated cell division.  Fetus development after transfer of blastocysts obtained from 72 hr culture under 5% O2 with BSA was significantly higher than those cultured under atmospheric oxygen without BSA.  However, the nuclear numbers of in vitro cultured blastocysts and fetus development after embryo transfer were still significantly lower than in vivo developed blastocysts, indicating the current culture condition is still suboptimal.},
 pages = {123--128},
 title = {Developmental Responses of 2-cell Embryos to Oxygen Tension and Bovine Serum Albumin in Wistar Rats},
 volume = {57},
 year = {2008}
}