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Rapid and simple method for quantitative evaluation of neurocyototoxic effects of radiation on developing medaka brain
https://repo.qst.go.jp/records/45279
https://repo.qst.go.jp/records/45279f22d0df2-7312-4092-a9d8-244ffe41de2a
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-10-02 | |||||
| タイトル | ||||||
| タイトル | Rapid and simple method for quantitative evaluation of neurocyototoxic effects of radiation on developing medaka brain | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
Yasuda, Takako
× Yasuda, Takako× Yoshimoto, Masami× Maeda, Keiko× Matsumoto, Atsuko× Maruyama, Kouichi× Ishikawa, Yuuji× 保田 隆子× 前田 圭子× 松本 厚子× 丸山 耕一× 石川 裕二 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We describe a novel method of rapid and quantitative evaluation of the degree of radiation-induced apoptosis in the developing brain of medaka (Oryzias latipes). Embryos at stage 28 were irradiated with 1, 2, 3.5, and 5 Gy of X-rays. Twenty to twenty-four hours after the irradiation, living embryos were stained with a vital dye, acridine orange (AO), for 1-2 h, and whole-mount brains were examined under an epifluorescence microscope. The numbers of AO-stained rosette-shaped nuclear clusters and AO-stained single nuclei increased in a dose-dependent manner in the optic tectum. Electron microscopy revealed that the AO-stained rosette-shaped nuclear clusters were the nuclei of aggregated apoptotic cells. We used the number of AO-stained rosette-shaped nuclear clusters/tectum as an index of the degree of radiation-induced brain cell death. The results showed that the number of rosette-shaped nuclear clusters/optic tectum in irradiated embryos exposed to higher than 2 Gy was highly significantly different from that in nonirradiated control embryos, whereas no difference was detected at 1 Gy. Thus, the threshold dose for brain cell death in medaka embryos is about 1 Gy and this dose is probably comparable to those for rodents and humans. The results show that medaka embryos are useful for quantitative evaluation of developmental neurocytotoxic effects of radiation. | |||||
| 書誌情報 |
Journal of Radiation Research 巻 49, 号 5, p. 533-540, 発行日 2008-09 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0449-3060 | |||||
